6.2.1.1: acetate-CoA ligase

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For detailed information about acetate-CoA ligase, go to the full flat file.

Reaction

Synonyms

ACAS, ACD, AceCS, AceCS1, AceCS2, Acetate thiokinase, Acetate--CoA ligase, acetate:CoA ligase (AMP-forming), Acetic thiokinase, Acetyl activating enzyme, Acetyl CoA ligase, Acetyl CoA synthase, acetyl CoA synthetase, acetyl coenzyme A synthase/carbon monoxide dehydrogenase, Acetyl coenzyme A synthetase, Acetyl-CoA synthase, Acetyl-CoA synthetase, acetyl-CoA synthetase 2, acetyl-CoA synthetase short-chain family member 2, Acetyl-coenzyme A synthase, acetyl-coenzyme A synthase/carbon monoxide dehydrogenase, acetyl-coenzyme A synthetase, ACS, ACS/CODH, ACS1, Acs1p, ACS2, Acs2p, AcsA, acsA1, AcsA2, AcsA3, ACSS2, Acyl-activating enzyme, Acyl-CoA synthetase, adenosine monophosphate-forming acetyl-CoA synthetase, ADP-forming acetyl-CoA synthetase, AF-ACS2, AMP acetyl-coenzyme A synthetase, AMP-Acs, AMP-dependent ACS, AMP-forming acetyl coenzyme A synthetase, AMP-forming acetyl-CoA synthetase, AMP-forming ACS, AMP-forming CoA ligase, carbon monoxide dehydrogenase/acety-coenzyme A synthase, carbon monoxide dehydrogenase/acetyl-CoA synthase, CODH/ACS, More, MT-ACS1, mtCODH/ACS, Mthe_1194, SeAcs, Short chain fatty acyl-CoA synthetase, Short-chain acyl-coenzyme A synthetase, Synthetase, acetyl coenzyme A

ECTree

     6 Ligases

         6.2 Forming carbon-sulfur bonds

             6.2.1 Acid-thiol ligases

                6.2.1.1 acetate-CoA ligase

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Results	in table
11	Activating Compound
37970	AA Sequence
3	Application
1	CAS Registry Number
32	Cloned(Commentary)
29	Cofactor
5	Crystallization (Commentary)
475	Disease/ Diagnostics
4	Expression
23	General Information
9	General Stability
65	Inhibitors
6	kcat/KM [mM/s]
3	Ki Value [mM]
165	KM Value [mM]
36	Localization
75	Metals/Ions
39	Molecular Weight [Da]
60	Natural Substrates/ Products (Substrates)
145	Organism
1	Oxidation Stability
27	Pathway
68	PDB ID
26	pH Optimum
11	pH Range
5	pI Value
7	Posttranslational Modification
74	Protein Variants
35	Purification (Commentary)
4	Reaction
1	Reaction Type
127	Reference
78	Source Tissue
18	Specific Activity [micromol/min/mg]
7	Storage Stability
212	Substrates and Products (Substrate)
47	Subunits
157	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
4	Temperature Range [°C]
6	Temperature Stability [°C]
73	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 6.2.1.1 - acetate-CoA ligase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 0.2 mM DTT, stable for at least one month

Cereibacter sphaeroides

-

553

-20°C, 0.5 M potassium phosphate, pH 7.5, 7 mM 2-mercaptoethanol and 0.5 mM EDTA, protein concentration above 5 mg/ml, no loss of activity after 6 months

Saccharomyces cerevisiae

-

554

-20°C, cannot be stored for more than 3 days without a considerable loss of activity

Penicillium chrysogenum

-

574

-20°C, stable for several months

Ovis aries

-

557

0-4°C, 20 mM Tris/Hcl, pH 8.0, 10 mM ATP, 5 mM MgCl2, 1 mM DTT or 5 mM 2-mercaptoethanol, 20% glycerol, stable for at least 1 month

Bradyrhizobium japonicum

-

568

4°C, 0.1 M buffer, pH 7.5, 3 mM mercaptoethanol, stable for several weeks

Acetobacter aceti

-

560

4°C, 20% w/v glycerol, stable for 1 month

Bos taurus

-

572