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6.2.1.1: acetate-CoA ligase

This is an abbreviated version!
For detailed information about acetate-CoA ligase, go to the full flat file.

Word Map on EC 6.2.1.1

Reaction

ATP
+
acetate
 +
CoA
=
AMP
 +
diphosphate
 +
acetyl-CoA

Synonyms

ACAS, ACD, AceCS, AceCS1, AceCS2, Acetate thiokinase, Acetate--CoA ligase, acetate:CoA ligase (AMP-forming), Acetic thiokinase, Acetyl activating enzyme, Acetyl CoA ligase, Acetyl CoA synthase, acetyl CoA synthetase, acetyl coenzyme A synthase/carbon monoxide dehydrogenase, Acetyl coenzyme A synthetase, Acetyl-CoA synthase, Acetyl-CoA synthetase, acetyl-CoA synthetase 2, acetyl-CoA synthetase short-chain family member 2, Acetyl-coenzyme A synthase, acetyl-coenzyme A synthase/carbon monoxide dehydrogenase, acetyl-coenzyme A synthetase, ACS, ACS/CODH, ACS1, Acs1p, ACS2, Acs2p, AcsA, acsA1, AcsA2, AcsA3, ACSS2, Acyl-activating enzyme, Acyl-CoA synthetase, adenosine monophosphate-forming acetyl-CoA synthetase, ADP-forming acetyl-CoA synthetase, AF-ACS2, AMP acetyl-coenzyme A synthetase, AMP-Acs, AMP-dependent ACS, AMP-forming acetyl coenzyme A synthetase, AMP-forming acetyl-CoA synthetase, AMP-forming ACS, AMP-forming CoA ligase, carbon monoxide dehydrogenase/acety-coenzyme A synthase, carbon monoxide dehydrogenase/acetyl-CoA synthase, CODH/ACS, More, MT-ACS1, mtCODH/ACS, Mthe_1194, SeAcs, Short chain fatty acyl-CoA synthetase, Short-chain acyl-coenzyme A synthetase, Synthetase, acetyl coenzyme A

ECTree

     6 Ligases
         6.2 Forming carbon-sulfur bonds
             6.2.1 Acid-thiol ligases
                6.2.1.1 acetate-CoA ligase

Cloned

Cloned on EC 6.2.1.1 - acetate-CoA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
10-deacetylbaccatin III 10beta-O-acetyltransferase (DBAT) from Taxus can catalyze the transfer of acetyl, propionyl or n-butyryl from CoA to the C10-hydroxyl of 10-deacetylbaccatin III. Escherichia coli JM109 are transformed to recombinantly express dbat, and this enzyme function is coupled to that of acetyl-CoA synthase expressed from and regulated by genes encoded on the bacterial chromosome
Taxus sp.
-
acetyl-CoA synthetase overexpression in Escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C41(DE3) cells
expression in Escherichia coli
expression in Escherichia coli, AF-ACS2
-
expression of alpha-subunit in Escherichia coli
-
expression of C-terminally His-tagged ACS in Escherichia coli strain BL21(DE3)
-
gene acs, recombinant expression of His-tagged wild-type and mutant enzymes in Salmonella enterica strain G2466
-
genes acdIa and acdIb encoding the alpha and beta subunits, expression in Escherichia coli strain BL21(DE3)
-
overexpression in Escherichia coli
production in HEK -293 cells
residues 72-713 of ACS are subcloned into expression vector pET28a and expressed in Escherichia coli