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Literature summary for 6.2.1.1 extracted from

  • Preston, G.G.; Wall, J.D.; Emerich, D.W.
    Purification and properties of acetyl-CoA synthetase from Bradyrhizobium japonicum bacteroids (1990), Biochem. J., 267, 179-183.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.1

General Stability

General Stability Organism
ATP and glycerol stabilize during storage at 0-4°C Bradyrhizobium japonicum

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
 Bradyrhizobium japonicum
dicarbonic acid diethyl ester
-
 Bradyrhizobium japonicum
diphosphate
-
 Bradyrhizobium japonicum
FMN
-
 Bradyrhizobium japonicum
long-chain acyl-CoA compounds strong Bradyrhizobium japonicum
NaCl concentration of 5-20 mM decrease the activity 20-25% Bradyrhizobium japonicum
pyridoxal 5'-phosphate
-
 Bradyrhizobium japonicum
pyruvate
-
 Bradyrhizobium japonicum
Short-chain CoA esters
-
 Bradyrhizobium japonicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.146
-
 acetate
-
 Bradyrhizobium japonicum
0.202
-
 CoA
-
 Bradyrhizobium japonicum
0.275
-
 ATP
-
 Bradyrhizobium japonicum
0.4
-
 ADP
-
 Bradyrhizobium japonicum
2.3
-
 dATP
-
 Bradyrhizobium japonicum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Km for CaCl2 is 1.2 mM Bradyrhizobium japonicum
Ca2+ can replace Mg2+ in activation, with 50% of the efficiency relative to MgCl2 Bradyrhizobium japonicum
Co2+ can replace Mg2+ in activation, 50% as effective as MgCl2, Km for CoCl2 is 0.2 mM Bradyrhizobium japonicum
K+ KCl increases the activity of the enzyme about 60% at 5 mM and 80% at 20 mM Bradyrhizobium japonicum
Mg2+ required Bradyrhizobium japonicum
Mg2+ inhibition at high concentrations where the metal is present as the free ion Bradyrhizobium japonicum
Mg2+ Km for MgCl2 is 0.3 mM Bradyrhizobium japonicum
Mn2+ can replace Mg2+ in activation Bradyrhizobium japonicum
Mn2+ Km for MnCl2 is 0.5 mM Bradyrhizobium japonicum
NH4+ increases the activity by 30% at 5 mM and 70% at 20 mM Bradyrhizobium japonicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 2 * 72000, SDS-PAGE Bradyrhizobium japonicum
150000
-
 gel filtration Bradyrhizobium japonicum

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bradyrhizobium japonicum

Source Tissue

Source Tissue Comment Organism Textmining
bacteroid from Glycine max L. cv. Williams inoculated with Bradyrhizobium japonicum Bradyrhizobium japonicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.1
-
-
 Bradyrhizobium japonicum

Storage Stability

Storage Stability Organism
0-4°C, 20 mM Tris/Hcl, pH 8.0, 10 mM ATP, 5 mM MgCl2, 1 mM DTT or 5 mM 2-mercaptoethanol, 20% glycerol, stable for at least 1 month Bradyrhizobium japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + acetate + CoA 20% of the activity relative to ATP Bradyrhizobium japonicum AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA
-
 Bradyrhizobium japonicum AMP + diphosphate + acetyl-CoA
-
 ?
ATP + propanoate + CoA very poor activity Bradyrhizobium japonicum AMP + diphosphate + propanoyl-CoA
-
 ?
dATP + acetate + CoA 30% of the activity relative to ATP Bradyrhizobium japonicum dAMP + diphosphate + acetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 72000, SDS-PAGE Bradyrhizobium japonicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bradyrhizobium japonicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 min, 20% loss of activity Bradyrhizobium japonicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Bradyrhizobium japonicum

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 7.5: about 50% of maximal activity, 10.0: about 70% of maximal activity Bradyrhizobium japonicum