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6.1.1.1: tyrosine-tRNA ligase

This is an abbreviated version!
For detailed information about tyrosine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.1

Reaction

ATP
+
L-tyrosine
 +
tRNATyr
=
AMP
 +
diphosphate
 +
L-tyrosyl-tRNATyr

Synonyms

CHOTyrRS, class I tyrosyl-tRNA synthetase, CYT-18, CYT-18 protein, dTyRS, hTyrRS, LdTyrRS, LMJF_14_1370, mini-tyrosyl-tRNA synthetase, mini-TyrRS, mitochondrial tyrosyl-tRNA synthetase, MjYRS, mt-TyrRS, mtTyrRS, NpAla TyrRS, p-BrPhe TyrRS, tRNATyr/tyrosyl-tRNA synthetase, Tyrosine translase, Tyrosine tRNA synthetase, Tyrosine--tRNA ligase, Tyrosine-transfer ribonucleate synthetase, Tyrosine-transfer RNA ligase, tyrosyl aminoacyl-tRNA synthetase, tyrosyl synthetase, tyrosyl tRNA synthetase, Tyrosyl--tRNA ligase, Tyrosyl-transfer ribonucleate synthetase, Tyrosyl-transfer ribonucleic acid synthetase, Tyrosyl-transfer RNA synthetase, Tyrosyl-tRNA ligase, Tyrosyl-tRNA synthetase, tyrosyl—tRNA synthetase, TyrRS, TyrRSs, TyrRZ, tyrS, TYS1, YARS, YRS, YTS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.1 tyrosine-tRNA ligase

Purification

Purification on EC 6.1.1.1 - tyrosine-tRNA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by ammonium sulfate precipitation and on a heparin-Sepharose column
by chromatography on Ni-nitrilotriacetic acid agarose
-
by utilizing the His-tag
catalytic domain (residues 1-322)
Cibacron Blue 3GA column chromatography
mutant DELTA424-669
-
on a Source 15Q-Sepharose anion-exchange column
-
recombinant C-terminal domain from Escherichia coli strain BL21(DE3) by cation exchange chromatography and gel filtration
-
recombinant C-terminally His6-tagged tyrosyl aminoacyl-tRNA synthetase from Escherichia coli by nickel affinity and ion exchange chromatography to homogeneity
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity, recombinant His-tagged mutant enzymes from Saccharomyces cerevisiae strain BY4742 by nickel affinity chromatography to over 90% purity
recombinant enzyme to near homogeneity
-
recombinant His-Tagged enzyme form Escherichia coli strain BL21(DE3), removal of the N-terminal His-tag
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag cleavage by 3C protease, and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21, 4.2fold
-
recombinant HIs-tagged enzyme from overexpression in Escherichia coli strain JM105
-
recombinant His-tagged full-length wild-type and mutant mitochondrial enzymes, and truncated mutant mt-TyrRS-DS4 mitochondrial enzyme from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3)
-
recombinant HIs-tagged wild-type and mutant F130S from strain JM109
-
recombinant His-tagged wild-type and mutant Y43G enzyme from Escherichia coli Y43G
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His6-tagged wild-type enzyme and site-specifically acetylated TyrRS variants from Escherichia coli strain BL21(DE3) cells
recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography, to homogeneity
recombinant thioredoxin-His-tagged enzyme splice variants from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant truncated enzyme SceTyrRS comprising residues 1-364
recombinant TyrRSfrom Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant wild-type and mutant His-tagged enzymes
-
synthetase 1-3
-
synthetase T1 and T2
-
using Ni-NTA chromatography
wild-type and mutant enzymes
-