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Literature summary for 6.1.1.1 extracted from

  • Kapoor, M.; Otero, F.J.; Slike, B.M.; Ewalt, K.L.; Yang, X.L.
    Mutational separation of aminoacylation and cytokine activities of human tyrosyl-tRNA synthetase (2009), Chem. Biol., 16, 531-539.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine some mammalian synthetases develop cytokine functions possibly linked to disease-causing mutations in tRNA synthetases Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
mutant human mini-TyrRS constructs are generated using QuikChange site-directed mutagenesis kit and using a plasmid encoding the gene for wild-type human mini-TyrRS as the template for PCR mutagenesis reactions, all proteins are expressed with a C-terminal His-tag to facilitate purification Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
mini-TyrRS_D173A binding pocket variant, retains cytokine function Homo sapiens
mini-TyrRS_TyrRS(ELQ) the surface helix encodes an ELR motif that functions like the ELR tripeptide in CXC cytokines, substitution of Arg93 to generate ELQ Homo sapiens
mini-TyrRS_TyrRS(EYR) the surface helix encodes an ELR motif that functions like the ELR tripeptide in CXC cytokines, substitution of Leu92 to generate EYR Homo sapiens
mini-TyrRS_TyrRS(NLR) the surface helix encodes an ELR motif that functions like the ELR tripeptide in CXC cytokines, substitution of Glu91 to generate NLR Homo sapiens
mini-TyrRS_Y39A binding pocket variant, retains cytokine function Homo sapiens
mini-TyrRS_Y39A/D173A binding pocket variant, retains cytokine function Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tyrosine + tRNATyr Homo sapiens
-
AMP + diphosphate + L-tyrosyl-tRNATyr
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P54577
-
-

Purification (Commentary)

Purification (Comment) Organism
by utilizing the His-tag Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HUVEC cell
-
Homo sapiens
-
PMN cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tyrosine + tRNATyr
-
Homo sapiens AMP + diphosphate + L-tyrosyl-tRNATyr
-
?

Synonyms

Synonyms Comment Organism
Tyrosyl-tRNA synthetase
-
Homo sapiens
TyrRS
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
aminoacylation assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
aminoacylation assay Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens