Application | Comment | Organism |
---|---|---|
medicine | some mammalian synthetases develop cytokine functions possibly linked to disease-causing mutations in tRNA synthetases | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
mutant human mini-TyrRS constructs are generated using QuikChange site-directed mutagenesis kit and using a plasmid encoding the gene for wild-type human mini-TyrRS as the template for PCR mutagenesis reactions, all proteins are expressed with a C-terminal His-tag to facilitate purification | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
mini-TyrRS_D173A | binding pocket variant, retains cytokine function | Homo sapiens |
mini-TyrRS_TyrRS(ELQ) | the surface helix encodes an ELR motif that functions like the ELR tripeptide in CXC cytokines, substitution of Arg93 to generate ELQ | Homo sapiens |
mini-TyrRS_TyrRS(EYR) | the surface helix encodes an ELR motif that functions like the ELR tripeptide in CXC cytokines, substitution of Leu92 to generate EYR | Homo sapiens |
mini-TyrRS_TyrRS(NLR) | the surface helix encodes an ELR motif that functions like the ELR tripeptide in CXC cytokines, substitution of Glu91 to generate NLR | Homo sapiens |
mini-TyrRS_Y39A | binding pocket variant, retains cytokine function | Homo sapiens |
mini-TyrRS_Y39A/D173A | binding pocket variant, retains cytokine function | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | Homo sapiens | - |
AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P54577 | - |
- |
Purification (Comment) | Organism |
---|---|
by utilizing the His-tag | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HUVEC cell | - |
Homo sapiens | - |
PMN cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | - |
Homo sapiens | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tyrosyl-tRNA synthetase | - |
Homo sapiens |
TyrRS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
aminoacylation assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
aminoacylation assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |