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Literature summary for 6.1.1.1 extracted from
- Tyrosyl-tRNA synthetase from wheat germ (1988), J. Biol. Chem., 263, 15277-15281.
General Stability
| General Stability | Organism |
|---|---|
| dilution has denaturing effect | Triticum aestivum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
tRNATyr | - |
Triticum aestivum |  |
| 0.0033 | - |
tyrosine | - |
Triticum aestivum | |
| 0.04 | - |
ATP | - |
Triticum aestivum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | activation up to a concentration of 200 mM, sharp decrease above | Triticum aestivum | |
| Mg2+ | optimum at 6 mM | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000, SDS-PAGE | Triticum aestivum |
| 110000 | - |
gel filtration | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + tyrosine + tRNATyr | Triticum aestivum | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
wheat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tyrosine + tRNATyr | - |
Triticum aestivum | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
| ATP + tyrosine + tRNATyr | - |
Triticum aestivum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000, SDS-PAGE | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Triticum aestivum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8.5 | less than 50% of maximal activity above and below | Triticum aestivum |
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Release 2023.1 (January 2023)
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