5.4.99.25: tRNA pseudouridine55 synthase
This is an abbreviated version!
For detailed information about tRNA pseudouridine55 synthase, go to the full flat file.
Word Map on EC 5.4.99.25
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5.4.99.25
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synthases
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pseudouridylation
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anticodon
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rna-guided
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5-fluorouridine
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srnps
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thumb
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snornas
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eukaryal
- 5.4.99.25
- synthases
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pseudouridylation
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anticodon
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rna-guided
- 5-fluorouridine
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srnps
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thumb
- snornas
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eukaryal
Reaction
Synonyms
aCBF5, Cbf5, Mj-Pus10, MjPus10, More, pfuCbf5, pseudouridine 55 synthase, pseudouridine synthase, pseudouridine synthase Pus10, pseudouridine synthase TruB, pseuduridine-55 synthase, PSI synthase, PSI synthase TruB, PSI55 synthase, PSI55 synthaseuB, psi55 tRNA pseudouridine synthase, psi55S, Pus10, Pus4, RNA pseudouridine synthase TruB, TR, tRNA pseudouridine 55 synthase, tRNA pseudouridine synthase, tRNA PSI 55 synthase, tRNA:pseudouridine-55 synthase, tRNA:PSI55 synthase, tRNA:PSI55-synthase, TruB, YNL292w
ECTree
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Turnover Number
Turnover Number on EC 5.4.99.25 - tRNA pseudouridine55 synthase
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additional information
additional information
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turnover numbers are determined for wild-type and mutant forms of yeast tRNAPhe. The 7 base T-loop can be expanded or contracted by 1 base and still retains activity, albeit with a 30fold reduction in kcat
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0.011
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19R
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0.012
tRNA uridine55
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19M
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0.11
tRNA uridine55
pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme
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0.11
tRNA uridine55
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A/C174A/C193A
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0.12
tRNA uridine55
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme
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0.16
tRNA uridine55
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C174A
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0.18
tRNA uridine55
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C193V
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0.23
tRNA uridine55
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20G
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0.26
tRNA uridine55
pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A
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0.52
tRNA uridine55
pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20L
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0.78
tRNA uridine55
pseudouridine synthase TruB with an 10-amino acid N-terminal truncation
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