5.4.99.25: tRNA pseudouridine55 synthase

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For detailed information about tRNA pseudouridine55 synthase, go to the full flat file.

Word Map on EC 5.4.99.25

5.4.99.25

synthases

pseudouridylation

anticodon

rna-guided

5-fluorouridine

srnps

thumb

snornas

eukaryal

Reaction

Synonyms

aCBF5, Cbf5, Mj-Pus10, MjPus10, More, pfuCbf5, pseudouridine 55 synthase, pseudouridine synthase, pseudouridine synthase Pus10, pseudouridine synthase TruB, pseuduridine-55 synthase, PSI synthase, PSI synthase TruB, PSI55 synthase, PSI55 synthaseuB, psi55 tRNA pseudouridine synthase, psi55S, Pus10, Pus4, RNA pseudouridine synthase TruB, TR, tRNA pseudouridine 55 synthase, tRNA pseudouridine synthase, tRNA PSI 55 synthase, tRNA:pseudouridine-55 synthase, tRNA:PSI55 synthase, tRNA:PSI55-synthase, TruB, YNL292w

ECTree

5 Isomerases

5.4 Intramolecular transferases

5.4.99 Transferring other groups

5.4.99.25 tRNA pseudouridine55 synthase

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Results	in table
6	Activating Compound
49363	AA Sequence
2	CAS Registry Number
13	Cloned(Commentary)
7	Crystallization (Commentary)
15	Disease/ Diagnostics
12	General Information
1	Inhibitors
10	kcat/KM [mM/s]
14	KM Value [mM]
2	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
31	Organism
5	pH Optimum
35	Protein Variants
9	Purification (Commentary)
7	Reaction
30	Reference
1	Source Tissue
23	Substrates and Products (Substrate)
3	Subunits
50	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
14	Turnover Number [1/s]

Cloned

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Cloned on EC 5.4.99.25 - tRNA pseudouridine55 synthase

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4 entries

cloned in an Escherichia coli expression vector downstream of a His6-tag. The resulting recombinant protein Pus4 is expressed at high level

Saccharomyces cerevisiae

P48567

648862

expressed in Escherichia coli as a His-tagged fusion protein

2 entries

gene PUS10, genotyping, phylogenetic analysis

2 entries

wild-type and mutant enzymes, expression in Escherichia coli. Wild-type enzyme can produce both pseudouridine54 and pseudouridine55 in Escherichia coli tRNAs

Methanocaldococcus jannaschii

Q60346

728787