5.4.99.25: tRNA pseudouridine55 synthase

This is an abbreviated version!
For detailed information about tRNA pseudouridine55 synthase, go to the full flat file.

Reaction

Synonyms

aCBF5, Cbf5, Mj-Pus10, MjPus10, More, pfuCbf5, pseudouridine 55 synthase, pseudouridine synthase, pseudouridine synthase Pus10, pseudouridine synthase TruB, pseuduridine-55 synthase, PSI synthase, PSI synthase TruB, PSI55 synthase, PSI55 synthaseuB, psi55 tRNA pseudouridine synthase, psi55S, Pus10, Pus4, RNA pseudouridine synthase TruB, TR, tRNA pseudouridine 55 synthase, tRNA pseudouridine synthase, tRNA PSI 55 synthase, tRNA:pseudouridine-55 synthase, tRNA:PSI55 synthase, tRNA:PSI55-synthase, TruB, YNL292w

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.99 Transferring other groups

                5.4.99.25 tRNA pseudouridine55 synthase

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Results	in table
6	Activating Compound
49363	AA Sequence
2	CAS Registry Number
13	Cloned(Commentary)
7	Crystallization (Commentary)
15	Disease/ Diagnostics
12	General Information
1	Inhibitors
10	kcat/KM [mM/s]
14	KM Value [mM]
2	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
31	Organism
5	pH Optimum
35	Protein Variants
9	Purification (Commentary)
7	Reaction
30	Reference
1	Source Tissue
23	Substrates and Products (Substrate)
3	Subunits
50	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
14	Turnover Number [1/s]

Crystallization

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Crystallization on EC 5.4.99.25 - tRNA pseudouridine55 synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

atomic-resolution crystal structure, TruB bound to RNA at 1.85 A resolution

Escherichia coli

P60340

650781

the TruB crystallization construct (tTruB), differs from full-length Escherichia coli TruB in having an N-terminal His-tag followed by residue 10 of the full-length protein. tTruB(D48N) is cocrystallized with [5-fluorouridine]-RNA. Initially, crystals are obtained by seeding with fragments of tTruB–RNA cocrystals. The resulting crystals are crushed and used as seeds for six subsequent sequential rounds of seeding into tTruB(D48N)–RNA complex crystallization experiments

Escherichia coli

P60340

663343

vapor-phase diffusion method, crystal structure of TruB apoenzyme in the absence of RNA. Comparison of the TruB apoenzyme from Escherichia coli and the RNA-bound forms of Thermotoga maritima TruB provides insight into the structural basis for RNA recognition and specificity

Escherichia coli

P60340

653587

sitting drop vapor diffusion method, structure of pseudouridine55 tRNA in two substrate-free forms, at 1.9 A resolution

Mycobacterium tuberculosis

P9WHP7

652542

crystal structure is diffracted to a resolution of 1.7 A. The crystal belong to space group P2, with unit-cell parameters a = 37.65, b = 78.09, c = 56.33 A , c = 102.05

Streptococcus pneumoniae

Q97QJ3

728913

crystal structure of the Y67F mutant in complex with a 5-fluorouridine-tRNA at 2.4 A resolution

Thermotoga maritima

Q9WZW0

661179

vapor-phase diffusion method, crystal structure of TruB in complex with 17-base stem-loop of RNA. Comparison of the TruB apoenzyme from Escherichia coli and the RNA-bound form of Thermotoga maritima TruB provides insight into the structural basis for RNA recognition and specificity

Thermotoga maritima

Q9WZW0

653587