5.4.99.25: tRNA pseudouridine55 synthase

This is an abbreviated version!
For detailed information about tRNA pseudouridine55 synthase, go to the full flat file.

Reaction

Synonyms

aCBF5, Cbf5, Mj-Pus10, MjPus10, More, pfuCbf5, pseudouridine 55 synthase, pseudouridine synthase, pseudouridine synthase Pus10, pseudouridine synthase TruB, pseuduridine-55 synthase, PSI synthase, PSI synthase TruB, PSI55 synthase, PSI55 synthaseuB, psi55 tRNA pseudouridine synthase, psi55S, Pus10, Pus4, RNA pseudouridine synthase TruB, TR, tRNA pseudouridine 55 synthase, tRNA pseudouridine synthase, tRNA PSI 55 synthase, tRNA:pseudouridine-55 synthase, tRNA:PSI55 synthase, tRNA:PSI55-synthase, TruB, YNL292w

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.99 Transferring other groups

                5.4.99.25 tRNA pseudouridine55 synthase

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Results	in table
6	Activating Compound
49363	AA Sequence
2	CAS Registry Number
13	Cloned(Commentary)
7	Crystallization (Commentary)
15	Disease/ Diagnostics
12	General Information
1	Inhibitors
10	kcat/KM [mM/s]
14	KM Value [mM]
2	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
31	Organism
5	pH Optimum
35	Protein Variants
9	Purification (Commentary)
7	Reaction
30	Reference
1	Source Tissue
23	Substrates and Products (Substrate)
3	Subunits
50	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
14	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 5.4.99.25 - tRNA pseudouridine55 synthase

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

aGar1 protein

Methanocaldococcus jannaschii

-

the aCbf5 alone can produce pseudouridine55 in tRNAs that contain the canonical 3’-CCA sequence. This activity is stimulated by aGar1

706710

-

aNop10 protein

Methanocaldococcus jannaschii

-

aCbf5 and aGar1 together can function as a tRNA pseudouridine55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae

706710

-

protein aGar1

Methanocaldococcus jannaschii

-

aCbf5 alone can also produce Y55 in tRNAs that contain the canonical 3'-CCA sequence and this activity is stimulated by aGar1

706710

-

protein aNOP10

2 entries

-

additional information

Pyrococcus furiosus

-

pfuCbf5 can modify tRNA uridine55, but not rRNA, in the absence of Gar1, Nop10 and L7Ae. The activity of pfuCbf5 toward tRNA uridine55 is enhanced in the presence of the accessory proteins, suggesting that a multi-protein Cbf5 complex may function in pseudouridine55 synthesis in Pyrococcus furiosus

705958

-

protein aNOP10

Methanocaldococcus jannaschii

-

aCbf5 and aGar1 together can function as a tRNA Y55 synthase in a guide RNA-independent manner. This activity is enhanced by aNop10, but not by L7Ae

706710

-

protein aNOP10

Pyrococcus abyssi

Q9V1A5

interaction with the pseudouridine synthase aCBF5 is required for the RNA-guided RNA:pseudouridine-synthase activity. The stable anchoring of aCBF5 to tRNAs relies on its PUA domain and the tRNA CCA sequence. Nonetheless, interaction of aNOP10 with aCBF5 can counterbalance the absence of the PUA domain or the CCA sequence and more generally helps the aCBF5 tRNA:pseudouridine55-synthase activity. Substitution of the aNOP10 residue Y14 by an alanine disturbs this activity, it only impairs mildly the RNA-guided activity. The opposite effect is observed for the aNOP10 variant H31A

705960

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