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Literature summary for 5.4.99.25 extracted from

  • Hoang, C.; Hamilton, C.S.; Mueller, E.G.; Ferre-D'Amare, A.R.
    Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain (2005), Protein Sci., 14, 2201-2206.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
the TruB crystallization construct (tTruB), differs from full-length Escherichia coli TruB in having an N-terminal His-tag followed by residue 10 of the full-length protein. tTruB(D48N) is cocrystallized with [5-fluorouridine]-RNA. Initially, crystals are obtained by seeding with fragments of tTruB–RNA cocrystals. The resulting crystals are crushed and used as seeds for six subsequent sequential rounds of seeding into tTruB(D48N)–RNA complex crystallization experiments Escherichia coli

Protein Variants

Protein Variants Comment Organism
D48N inactive mutant enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
tRNA uridine55 pseudouridine synthase TruB with an 10-amino acid N-terminal truncation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P60340
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine55
-
Escherichia coli tRNA pseudouridine55
-
?

Synonyms

Synonyms Comment Organism
pseudouridine synthase TruB
-
Escherichia coli
PSI synthase TruB
-
Escherichia coli
TruB
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.78
-
tRNA uridine55 pseudouridine synthase TruB with an 10-amino acid N-terminal truncation Escherichia coli