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5.3.3.12: L-dopachrome isomerase

This is an abbreviated version!
For detailed information about L-dopachrome isomerase, go to the full flat file.

Word Map on EC 5.3.3.12

Reaction

L-Dopachrome
=
5,6-Dihydroxyindole-2-carboxylate

Synonyms

brown (b) locus protein, D-dopachrome isomerase, D-dopachrome tautomerase, D-DT, DCF, DCT, DDT, dopachrome conversion factor, dopachrome delta-isomerase, dopachrome DELTA7-DELTA2-isomerase, dopachrome oxidoreductase, dopachrome tautomerase, dopachrome-rearranging enzyme, isomerase, dopachrome DELTA-, L-dopachchrome-methyl ester tautomerase, macrophage inhibitory factor, Macrophage migration inhibitory factor, macrophage migration-inhibitory factor, MIF, Mif-2, SLATY locus protein, TRP-2, TRP2, tyrosine-related protein 2

ECTree

     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.3 Transposing C=C bonds
                5.3.3.12 L-dopachrome isomerase

Engineering

Engineering on EC 5.3.3.12 - L-dopachrome isomerase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
P1G
inactive
G486R
-
slaty light mutation, 28fold reduction in enzyme activity, sliding of transmembrane domain towards N-terminus of protein thus interfering with the active site. Increase in pheomelanin and reduction in eumelanin produced by melanocytes in culture
P1G
-
generation of knockout mice in which the endogenous mif gene is replaced by one encoding a tautomerase-null, Pro1-Gly1 MIF protein, i.e. P1G-MIF, which is P1G-MIF is catalytically completely inactive, but maintains significant, albeit reduced, binding to its cell surface receptor CD74 and to the intracellular binding protein JAB1/CSN5. Mutant mice show a phenotype in assays of growth control and tumor induction that is intermediate between those of the wild type mif+/+ and complete MIF deficiency mif-/-, overview. Reduced development of benzo[alpha]pyrene-induced skin tumors in mif-deficient mice
R194Q
-
slaty mutation, 3fold reduction in enzyme activity compared to wild-type. Increase in pheomelanin and reduction in eumelanin produced by melanocytes in culture
additional information