Literature summary for 5.3.3.12 extracted from

Cross, J.V.; Rady, J.M.; Foss, F.W.; Lyons, C.E.; Macdonald, T.L.; Templeton, D.J.
Nutrient isothiocyanates covalently modify and inhibit the inflammatory cytokine macrophage migration inhibitory factor (MIF) (2009), Biochem. J., 423, 315-321.

Search for more literature for 5.3.3.12

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of MIF in HeLa cells and in Escherichia coli strain BL21(DE3)	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	preparation of biotin-isothiocyanate-labeled enzyme, proteomic screening in recombinant HeLa cells and identification of the modification site by mass spectrometry	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Isothiocyanate	inhibits tautomerase activity of MIF. Modification and inhibition of MIF is not involved in the induction of phase 2 gene expression in response to isothiocyanate treatment	Homo sapiens
phenethylisothiocyanate	inhibits tautomerase activity of MIF	Homo sapiens
sulforaphane	inhibits tautomerase activity of MIF	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	MIF is a 12 kDa protein with an enzymatic keto-enol tautomerase activityMIF is a homotrimeric multifunctional proinflammatory cytokine. Binding of MIF to its receptor, CD74, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-3,4-dihydroxyphenylalanine methyl ester	i.e. L-3,4-dihydroxyphenylalanine methyl ester hydrochloride, the catalytic residue is N-terminal Pro1	Homo sapiens	methyl 3-(3,4-dioxocyclohexa-1,5-dien-1-yl)-L-alaninate	-	r
additional information	MIF is a 12 kDa protein with an enzymatic keto-enol tautomerase activityMIF is a homotrimeric multifunctional proinflammatory cytokine. Binding of MIF to its receptor, CD74, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
D-dopachrome tautomerase	-	Homo sapiens
Macrophage migration inhibitory factor	-	Homo sapiens
MIF	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.001	0.002	-	Homo sapiens	sulforaphane
0.005	0.006	-	Homo sapiens	phenethylisothiocyanate

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Release 2023.1 (January 2023)