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4.2.3.27: isoprene synthase

This is an abbreviated version!
For detailed information about isoprene synthase, go to the full flat file.

Word Map on EC 4.2.3.27

Reaction

dimethylallyl diphosphate
=
isoprene
+
diphosphate

Synonyms

isoprene synthase, ISPS, ispS3, PcISPS, PnISPS

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.27 isoprene synthase

General Stability

General Stability on EC 4.2.3.27 - isoprene synthase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
endogenous isoprene emission of plants plays a protective role against three abiotic stresses (heat, combined heat/light and ozone-induced oxidative stress)
glycerol stabilizes the enzyme in the early stages of purification, PEG in the late stages
-
isoprene biosynthesis by ISPS is relatively unaffected by drought
the enzyme activity is very labile and only reproducibly detected when suitable protectants are used in the extraction buffer, especially PMSF is highly stabilizing
-