Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.27 extracted from

  • Sivy, T.L.; Shirk, M.C.; Fall, R.
    Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis (2002), Biochem. Biophys. Res. Commun., 294, 71-75.
    View publication on PubMed

General Stability

General Stability Organism
the enzyme activity is very labile and only reproducibly detected when suitable protectants are used in the extraction buffer, especially PMSF is highly stabilizing Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline weak, 20% inhibition at 10 mM Bacillus subtilis
Ca2+ inhibitory at higher concentrations Bacillus subtilis
Co2+ inhibits at 1 mM Bacillus subtilis
EDTA 40% inhibition at 1 mM, 70% inhibition at 10 mM Bacillus subtilis
Fe2+ inhibits at 1 mM Bacillus subtilis
Mg2+ activates at low concentration below 0.1 mM, inhibits at higher concentrations Bacillus subtilis
Mn2+ activates at low concentration below 0.1 mM, inhibits at higher concentrations Bacillus subtilis
additional information no inhibition by EGTA Bacillus subtilis
Zn2+ inhibits at 1 mM Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates at low concentration below 0.1 mM, inhibits at higher concentrations Bacillus subtilis
Mn2+ activates at low concentration below 0.1 mM, inhibits at higher concentrations Bacillus subtilis
additional information the enzyme requires low levels of divalent cations Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate Bacillus subtilis in presence of a divalent cation isoprene + diphosphate
-
ir
dimethylallyl diphosphate Bacillus subtilis 6051 in presence of a divalent cation isoprene + diphosphate
-
ir
additional information Bacillus subtilis the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview ?
-
?
additional information Bacillus subtilis 6051 the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 6051
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by anion exchange chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate in presence of a divalent cation Bacillus subtilis isoprene + diphosphate
-
ir
dimethylallyl diphosphate in presence of a divalent cation Bacillus subtilis 6051 isoprene + diphosphate
-
ir
additional information the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview Bacillus subtilis ?
-
?
additional information the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview Bacillus subtilis 6051 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
pH profile Bacillus subtilis