General Stability | Organism |
---|---|
the enzyme activity is very labile and only reproducibly detected when suitable protectants are used in the extraction buffer, especially PMSF is highly stabilizing | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | weak, 20% inhibition at 10 mM | Bacillus subtilis | |
Ca2+ | inhibitory at higher concentrations | Bacillus subtilis | |
Co2+ | inhibits at 1 mM | Bacillus subtilis | |
EDTA | 40% inhibition at 1 mM, 70% inhibition at 10 mM | Bacillus subtilis | |
Fe2+ | inhibits at 1 mM | Bacillus subtilis | |
Mg2+ | activates at low concentration below 0.1 mM, inhibits at higher concentrations | Bacillus subtilis | |
Mn2+ | activates at low concentration below 0.1 mM, inhibits at higher concentrations | Bacillus subtilis | |
additional information | no inhibition by EGTA | Bacillus subtilis | |
Zn2+ | inhibits at 1 mM | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates at low concentration below 0.1 mM, inhibits at higher concentrations | Bacillus subtilis | |
Mn2+ | activates at low concentration below 0.1 mM, inhibits at higher concentrations | Bacillus subtilis | |
additional information | the enzyme requires low levels of divalent cations | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | Bacillus subtilis | in presence of a divalent cation | isoprene + diphosphate | - |
ir | |
dimethylallyl diphosphate | Bacillus subtilis 6051 | in presence of a divalent cation | isoprene + diphosphate | - |
ir | |
additional information | Bacillus subtilis | the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview | ? | - |
? | |
additional information | Bacillus subtilis 6051 | the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis 6051 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially by anion exchange chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | in presence of a divalent cation | Bacillus subtilis | isoprene + diphosphate | - |
ir | |
dimethylallyl diphosphate | in presence of a divalent cation | Bacillus subtilis 6051 | isoprene + diphosphate | - |
ir | |
additional information | the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview | Bacillus subtilis | ? | - |
? | |
additional information | the enzyme activity parallels fluctuations of isoprene release during growth of Bacillus subtilis and is highly regulated, overview | Bacillus subtilis 6051 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Bacillus subtilis |