Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.2.3.27: isoprene synthase

This is an abbreviated version!
For detailed information about isoprene synthase, go to the full flat file.

Word Map on EC 4.2.3.27

Reaction

dimethylallyl diphosphate
=
isoprene
 +
diphosphate

Synonyms

isoprene synthase, ISPS, ispS3, PcISPS, PnISPS

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.27 isoprene synthase

Inhibitors

Inhibitors on EC 4.2.3.27 - isoprene synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
weak, 20% inhibition at 10 mM
Cu2+
-
about 85% residual activity at 1 mM
diethyldicarbonate
-
34-59% inhibition at 1.1 mM, dimethylally diphosphate and Mg2+ protect the soluble enzyme, but not the solubilized thylakoid membrane isozyme
dimethylallyl diphosphate
-
substrate inhibition above 10 mM
diphosphate
-
69-100% inhibition
Mn2+
-
activates at low concentration below 0.1 mM, inhibits at higher concentrations
N-ethylmaleimide
-
pre-incubation of the partially-purified enzyme with 5 mM N-ethylmaleimide results in 66% inhibition of activity
NEM
-
complete inactivation at 1.1 mM, dimethylally diphosphate and Mg2+ protect the isozyme partially
Ni2+
-
about 80% residual activity at 1 mM
Ozone
Phenylglyoxal
-
50 mM, complete inactivation, dimethylally diphosphate and Mg2+ protect the isozyme partially
additional information
-