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Literature summary for 4.2.3.27 extracted from
- Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere (1995), J. Biol. Chem., 270, 13010-13016.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is positively regulated by light | Populus tremuloides |
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes the enzyme in the early stages of purification, PEG in the late stages | Populus tremuloides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dimethylallyl diphosphate | substrate inhibition above 10 mM | Populus tremuloides |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
dimethylallyl diphosphate | - |
Populus tremuloides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Populus tremuloides | |
| Mn2+ | can substitute for Mg2+ | Populus tremuloides | |
| additional information | the enzyme is strictly dependent on divalent cations, no activity with Zn2+ and Ca2+ | Populus tremuloides |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
1 * 58000 + 1 * 63000, SDS-PAGE | Populus tremuloides |
| 63000 | - |
1 * 58000 + 1 * 63000, SDS-PAGE | Populus tremuloides |
| 98000 | 137000 | gel filtration | Populus tremuloides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate | Populus tremuloides | - |
isoprene + diphosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus tremuloides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 4000fold from leaves to homogeneity, ammonium sulfate fractionation of cell-free supernatant, dialysis, ion exchange chromatography, ultrafiltration, and gel filtration | Populus tremuloides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Populus tremuloides | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.521 | - |
purified enzyme | Populus tremuloides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate | - |
Populus tremuloides | isoprene + diphosphate | - |
ir | |
| dimethylallyl diphosphate | - |
Populus tremuloides | isoprene + diphosphate | product stoichiometry determination, isoprene is volatile 2-methyl-1,3-butadiene emitted by the plant into the atmosphere, where it plays an important role, the enzyme is positively regulated by light | ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 58000 + 1 * 63000, SDS-PAGE | Populus tremuloides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
assay at | Populus tremuloides |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
dimethylallyl diphosphate | - |
Populus tremuloides | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Populus tremuloides |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Populus tremuloides | isoelectric focusing | - |
4.7 |
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