Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.2.3.27: isoprene synthase

This is an abbreviated version!
For detailed information about isoprene synthase, go to the full flat file.

Word Map on EC 4.2.3.27

Reaction

dimethylallyl diphosphate
=
isoprene
 +
diphosphate

Synonyms

isoprene synthase, ISPS, ispS3, PcISPS, PnISPS

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.27 isoprene synthase

Cloned

Cloned on EC 4.2.3.27 - isoprene synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Camelina sativa
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli XL1-Blue
expression analysis under different atmospheric conditions, overview, expression of the His-tagged ISPS in Escherichia coli strain BL21(DE3)
-
expression in Escherichia coli
gene ispS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, expression as GFP-fusion protein in onion peels and tobacco leaves, transformations by particle bombardement
gene ispS, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme with or without leader sequence in Escherichia coli strain TG1
gene ispS, expression of N-terminally or C-terminally His-tagged enzyme without plastidial targeting sequence in Escherichia coli strain TG1
-
His-tagged version expressed in Escherichia coli, after codon-use optimization expressed in Synechocystis sp. PCC6803
into the pET28a vector for production of a polyclonal primary antibody
-
into the pQE50 vector for expression in Escherichia coli, into the binary vector pBinAR for transformation of Agrobacterium tumefaciens and generating transgenic Arabidopsis thaliana plants
-
the plasmid pGWB2-PaIspS is introduced via Agrobacterium tumefaciens into Arabidopsis plants
transformation of tobacco with an isoprene synthase gene extracted from poplar, obtained plants emitting isoprene at levels comparable to a naturally emitting species, full-length and truncated isoprene synthase cDNAs are amplified