General Stability | Organism |
---|---|
isoprene biosynthesis by ISPS is relatively unaffected by drought | Populus nigra |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | When photosynthesis is completely inhibited by a 35-day drought stress, the isoprene emission of plants grown at 25°C and 35°C is strongly inhibited but still measurable, averaging 7.5 nmol/qm/sec. After a 3-day re-watering phase, the isoprene emission rate recovers in plants grown at both 25°C and 35°C. | Populus nigra |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Populus nigra | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | included in assay medium | Populus nigra |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | Populus nigra | - |
isoprene + diphosphate | isoprene is an important molecule for ameliorating abiotic stresses | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus nigra | A0PFK2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Populus nigra | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
A much higher PnISPS activity sustains high rates of isoprene emission in plants grow at 35°C before drought stress. Large reductions of PnISPS activity have no relevant effect on isoprene emission in conditions of mild drought stress. The PnISPS activities of leaves grown at 35°C do not recover the original rates once drought stress is relieved. | Populus nigra |
additional information | - |
ISPS activity decreases before isoprene emission during drought development, indicating a tighter control of the emission at a transcriptional or post-transcriptional level under moderate drought stress, at 25°C and 35°C. | Populus nigra |
additional information | - |
PnISPS activity is generally related to isoprene emission rates along the drought treatment | Populus nigra |
additional information | - |
Prior to the imposition of drought stress, plants grown at 35°C show much higher PnISPS activities than plants grown at 25°C. Drought stress negatively influences PnISPS activity, especially in plants grow at 35°C. In these plants, PnISPS activity starts to decrease when drought stress is mild and only partially recovered after re-watering. In plants grown at 25°C, the inhibition of PnISPS activity occurred more slowly and only became evident when the stress is severe. At 25°C, PnISPS activity recovers rapidly and completely upon re-watering. | Populus nigra |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | - |
Populus nigra | isoprene + diphosphate | isoprene is an important molecule for ameliorating abiotic stresses | ? |
Synonyms | Comment | Organism |
---|---|---|
isoprene synthase | - |
Populus nigra |
ISPS | - |
Populus nigra |
PnISPS | - |
Populus nigra |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Populus nigra |