4.2.3.27: isoprene synthase

This is an abbreviated version!
For detailed information about isoprene synthase, go to the full flat file.

isoprene synthase, ISPS, ispS3, PcISPS, PnISPS

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Results	in table
7	Activating Compound
23	AA Sequence
8	Application
1	CAS Registry Number
15	Cloned(Commentary)
1	Crystallization (Commentary)
1	Expression
4	General Stability
38	Inhibitors
1	kcat/KM [mM/s]
14	KM Value [mM]
19	Localization
35	Metals/Ions
12	Molecular Weight [Da]
39	Natural Substrates/ Products (Substrates)
47	Organism
1	Oxidation Stability
6	Pathway
4	PDB ID
20	pH Optimum
7	pH Range
1	pH Stability
1	pI Value
2	Posttranslational Modification
14	Purification (Commentary)
2	Reaction
46	Reference
37	Source Tissue
32	Specific Activity [micromol/min/mg]
2	Storage Stability
52	Substrates and Products (Substrate)
10	Subunits
43	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
7	Temperature Range [°C]
3	Temperature Stability [°C]
6	Turnover Number [1/s]

Storage Stability on EC 4.2.3.27 - isoprene synthase

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-70°C, partially purified enzyme, 40-55% ammonium sulfate fraction, in phosphate buffered saline, pH 7.4, 1mM DTT, stable for at least 1 month

-80°C, 30-60°C ammonium sulfate fraction, resuspended in 50 mM Tris-HCl, pH 8.5, 20 mM MgCl2, 5% glycerol, 2 mM DTT, stable