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Literature summary for 4.2.3.27 extracted from
- Enzymatic synthesis of isoprene from dimethylallyl diphosphate in aspen leaf extracts (1991), Plant Physiol., 97, 1588-1591.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Populus tremuloides |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate | Populus tremuloides | - |
isoprene + diphosphate | - |
ir | |
| additional information | Populus tremuloides | the acid-catalyzed non-enzymatic conversion of dimethylallyl diphosphate is negligible at physiologic pH | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus tremuloides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially from leaves by ammonium sulfate fractionation | Populus tremuloides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Populus tremuloides | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, partially purified enzyme, 40-55% ammonium sulfate fraction, in phosphate buffered saline, pH 7.4, 1mM DTT, stable for at least 1 month | Populus tremuloides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate | - |
Populus tremuloides | isoprene + diphosphate | - |
ir | |
| dimethylallyl diphosphate | - |
Populus tremuloides | isoprene + diphosphate | i.e. 2-methyl-1,3-butadiene, volatile product, product determination by gas chromatography | ir | |
| additional information | the acid-catalyzed non-enzymatic conversion of dimethylallyl diphosphate is negligible at physiologic pH | Populus tremuloides | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Populus tremuloides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Populus tremuloides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | - |
Populus tremuloides |
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Release 2023.1 (January 2023)
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