Literature summary for 4.2.3.27 extracted from
Lehning, A.; Zimmer, I.; Steinbrecher, R.; Bruggemann, N.; Schnitzler, J.P.
Isoprene synthase activity and its relation to isoprene emission in Quercus robur L. leaves (1999), Plant Cell Environ., 22, 495-504.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
additional information |
enzyme activity is induced by light, positive regulation |
Quercus robur |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Mg2+ |
dependent on |
Quercus robur |
|
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
dimethylallyl diphosphate |
Quercus robur |
the enzyme is positively regulated by light |
isoprene + diphosphate |
isoprene is emitted into the atmosphere following a diurnal cycle in vivo |
ir |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Quercus robur |
- |
penduculate oak |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
native enzyme partially from leaves by ammonium sulfate fractionation |
Quercus robur |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
leaf |
- |
Quercus robur |
- |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
additional information |
- |
Quercus robur is a strong emitter of isoprene, measurements of three trees over three days |
Quercus robur |
0.333 |
- |
partially purified enzyme |
Quercus robur |
Storage Stability
Storage Stability |
Organism |
-80°C, 30-60°C ammonium sulfate fraction, resuspended in 50 mM Tris-HCl, pH 8.5, 20 mM MgCl2, 5% glycerol, 2 mM DTT, stable |
Quercus robur |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
dimethylallyl diphosphate |
- |
Quercus robur |
isoprene + diphosphate |
i.e. 2-methyl-1,3-butadiene, product determination by gas chromatography |
ir |
|
dimethylallyl diphosphate |
the enzyme is positively regulated by light |
Quercus robur |
isoprene + diphosphate |
isoprene is emitted into the atmosphere following a diurnal cycle in vivo |
ir |
|
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
50 |
- |
- |
Quercus robur |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
15 |
50 |
exponential increase of activity from 15°C to 40°C, optimum activity at 50°C, inactivation at 60°C |
Quercus robur |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7.3 |
- |
first optimum |
Quercus robur |
7.7 |
- |
second optimum |
Quercus robur |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
6.3 |
9.2 |
sharp decrease in activity below pH 6.3 |
Quercus robur |