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4.2.3.19: ent-kaurene synthase

This is an abbreviated version!
For detailed information about ent-kaurene synthase, go to the full flat file.

Word Map on EC 4.2.3.19

Reaction

ent-copalyl diphosphate
=
ent-kaurene
 +
diphosphate

Synonyms

(-)-ent-kaurene synthase, AtKS, bifunctional CPS/KS, BjKS, blr2150, class I diTPS ent-kaurene synthase, copalyl diphosphate synthase/ent-kaurene synthase, copalyl diphosphate/kaurene synthase, CPS/KS, ent-beyerene synthase, ent-copalyl/ent-kaurene synthase, ent-copalyldiphosphate synthase/ent-kaurene synthase, ent-kaur-16-ene synthase, ent-kaurene synthase, ent-kaurene synthase B, ent-kaurene synthase like 2, ent-kaurene synthetase B, ent-KS, FfCPS/KS, kaurene synthase, KS, KS(-like) enzyme, KS(L), KS1, KS1-1, KS1A, ks2L, ks3L, KSL1, KSL2, KSL5, KSL6, More, MpKS, PgKS, PHYPA_009773, PpCPS/KS, PpKS, RcKS(L), RcKS1, RcKSL1, RcKSL2, RCOM_0823080, SdKS, SmKS, TPS4

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.19 ent-kaurene synthase

Cloned

Cloned on EC 4.2.3.19 - ent-kaurene synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
as a GST-fusion protein for expression in Escherichia coli
DNA and amino acid sequence determination of genes KS1-9, chromosomal localizations, gene KS9 is a pseudogene, phylogenetic analysis, overview
-
expressed in Escherichia coli as fusion protein with maltose-binding protein, enzyme exclusively shows EC 4.2.3.19 activity
-
expressed in Escherichia coli BL21 (DE3) and MG1655 cells
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21DE-C41 cells
expressed in Escherichia coli BL21DE3-C41 cells
-
expressed in Escherichia coli C41 (DE3) cells
expressed in Escherichia coli C41 cells
expressed in Escherichia coli JM109 as glutathione-S-transferase fusion protein, enzyme shows EC 4.2.3.19 and EC 5.5.1.13 activity
expressed in Escherichia coli JM109, enzyme shows EC 4.2.3.19 and EC 5.5.1.13 activity
expressed in Escherichia coli M15(DE3) cells
expressed in Escherichia coli OverExpress C41 cells
expressed in Escherichia coli strain C41
-
expressed in Escherichia coli strain Top10F-
expressed in Escherichia coli Top10 cells
gene KS, phylogenetic tree, quantitative real-time PCR expression analysis, functional recombinant expression in Escherichia coli strain C41(DE3), GC-MS analysis of diterpene products from hexane extracts of recombinant Escherichia coli harboring the pSdGGeC and pSdKS vectors
gene KS1, a synthetic ent-kaurene module, expressing Stevia rebaudiana CPPS and KS, and Rhodobacter sphaeroides GGPPS (crtE gene), is constructed for ent-kaurene production in diverse Escherichia coli strains, best in strain MG1655
gene PHYPA_009773, quantitative real-time PCR and semiquantitative RT-PCR enzyme expression analysis
gene PHYPA_009773, recombinant expression in Escherichia coli strain C41, transient expression of PpCPSKS with the suppressor of silencing P19 in leafs of 4-weeks-old Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL-1 mediated transformation
in Escherichia coli as glutathione-S-transferase fusion protein
into the pTD1 vector as a fusion of copalyl diphosphate synthase and ent-kaurene synthase for in vitro expression
isozyme determination and phylogenetic analysis and tree, sequence comparisons, overview. Synthetic RcKS(L) isozymes are truncated to remove the N-terminal plastid-directing transit peptide sequences, they are individually subcloned into compatible expression vectors and each coexpressed with either the geranylgeranyl phosphate synthase (GGPS), or the GGPS along with a CPS (EC 5.5.1.13) for recombinant expression in Escherichia coli
overexpression of CPS and KS in transgenic Arabidopsis thaliana plants, expression in Escherichia coli
-
recombinant expression of enzyme mutant H212A in Escherichia coli
recombinant expression of enzyme mutant H302A in Escherichia coli
recombinant expression of thioredoxin- and His-tagged wild-type and mutant enzymes with or without selenium-methionine labeling in Escherichia coli strain BL21trxB (DE3)
sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain C41(DE3)
sequence comparisons, recombinant expression of wild-type enzyme in Escherichia coli strain C41(DE3)
SmKS DNA and amino acid sequence determination and analysis of full-length enzyme from cDNA, sequence comparisons and phylogenetic analysis and tree, recombinant enzyme expression in Saccharomyces cerevisiae strain BY-T20