4.2.3.19: ent-kaurene synthase

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For detailed information about ent-kaurene synthase, go to the full flat file.

Reaction

Synonyms

(-)-ent-kaurene synthase, AtKS, bifunctional CPS/KS, BjKS, blr2150, class I diTPS ent-kaurene synthase, copalyl diphosphate synthase/ent-kaurene synthase, copalyl diphosphate/kaurene synthase, CPS/KS, ent-beyerene synthase, ent-copalyl/ent-kaurene synthase, ent-copalyldiphosphate synthase/ent-kaurene synthase, ent-kaur-16-ene synthase, ent-kaurene synthase, ent-kaurene synthase B, ent-kaurene synthase like 2, ent-kaurene synthetase B, ent-KS, FfCPS/KS, kaurene synthase, KS, KS(-like) enzyme, KS(L), KS1, KS1-1, KS1A, ks2L, ks3L, KSL1, KSL2, KSL5, KSL6, More, MpKS, PgKS, PHYPA_009773, PpCPS/KS, PpKS, RcKS(L), RcKS1, RcKSL1, RcKSL2, RCOM_0823080, SdKS, SmKS, TPS4

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.19 ent-kaurene synthase

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Results	in table
5	Activating Compound
74	AA Sequence
1	CAS Registry Number
42	Cloned(Commentary)
1	Crystallization (Commentary)
3	Expression
59	General Information
2	General Stability
6	IC50 Value
36	Inhibitors
4	KM Value [mM]
20	Localization
16	Metals/Ions
17	Molecular Weight [Da]
65	Natural Substrates/ Products (Substrates)
92	Organism
4	Pathway
15	pH Optimum
1	Posttranslational Modification
48	Protein Variants
23	Purification (Commentary)
12	Reaction
4	Reaction Type
67	Reference
40	Source Tissue
4	Specific Activity [micromol/min/mg]
2	Storage Stability
122	Substrates and Products (Substrate)
5	Subunits
103	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 4.2.3.19 - ent-kaurene synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant wild-type enzyme in apo form or in complex with inhibitor BPH-629, mutant D75C in complex with substrate ent-copalyl diphosphate, sitting drop vapor diffusion method, mixing of 6.4 mg/ml protein in 25 mM Tris, pH 7.5, and 150 mM NaCl with reservoir solution containing 0.7 M ammonium tartrate dibasic, pH 7.0 and 2-4% w/v PEG 4000 for the wild-type enzyme, for the inhibitor BPH-629 complex, the enzyme solution also contains 1 mM MgCl2, 10 mM DTT, and 2.5 mM BPH-629, for the substrate complex, the D75C mutant crystal is prepared under the same crystallization conditions, then soaked with the cryo-protectant containing 5 mM ent-copalyl diphosphate for 5 hours, 2-3 days, room temperature, X-ray diffraction structure determination and analysis at 1.80-2.34 A resolution

Bradyrhizobium diazoefficiens

Q45222

749301