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4.2.3.19: ent-kaurene synthase

This is an abbreviated version!
For detailed information about ent-kaurene synthase, go to the full flat file.

Word Map on EC 4.2.3.19

Reaction

ent-copalyl diphosphate
=
ent-kaurene
 +
diphosphate

Synonyms

(-)-ent-kaurene synthase, AtKS, bifunctional CPS/KS, BjKS, blr2150, class I diTPS ent-kaurene synthase, copalyl diphosphate synthase/ent-kaurene synthase, copalyl diphosphate/kaurene synthase, CPS/KS, ent-beyerene synthase, ent-copalyl/ent-kaurene synthase, ent-copalyldiphosphate synthase/ent-kaurene synthase, ent-kaur-16-ene synthase, ent-kaurene synthase, ent-kaurene synthase B, ent-kaurene synthase like 2, ent-kaurene synthetase B, ent-KS, FfCPS/KS, kaurene synthase, KS, KS(-like) enzyme, KS(L), KS1, KS1-1, KS1A, ks2L, ks3L, KSL1, KSL2, KSL5, KSL6, More, MpKS, PgKS, PHYPA_009773, PpCPS/KS, PpKS, RcKS(L), RcKS1, RcKSL1, RcKSL2, RCOM_0823080, SdKS, SmKS, TPS4

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.19 ent-kaurene synthase

Engineering

Engineering on EC 4.2.3.19 - ent-kaurene synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
I368A
the mutant produces ent-pimara-8(14),15-diene, ent-kaur-16-ene, 8alpha-hydroxy-ent-pimara-15-ene, and ent-pimara-7,15-diene from ent-copalyl diphosphate
I368S
the mutant produces ent-pimara-8(14),15-diene and 8alpha-hydroxy-ent-pimara-15-ene from ent-copalyl diphosphate
I368T
the mutant produces ent-pimara-8(14),15-diene from ent-copalyl diphosphate
I368V
the mutant produces ent-kaur-16-ene from ent-copalyl diphosphate
I638A
site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, the mutant produces a mixture of four products, pedominantly ent-pimara-8(14),15-diene, and a small amount of the double bond isomer ent-pimara-7,15-diene, and variable amounts of ent-kaur-16-ene, as well as substantial amounts of 8a-hydroxy-ent-pimar-15-ene
I638S
site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, the mutant produces a mixture of four products, pedominantly ent-pimara-8(14),15-diene, and a small amount of the double bond isomer ent-pimara-7,15-diene, and variable amounts of ent-kaur-16-ene, as well as substantial amounts of 8a-hydroxy-ent-pimar-15-ene
I638T
site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, the mutant produces a mixture of four products, pedominantly ent-pimara-8(14),15-diene, and small amounts of the double bond isomer ent-pimara-7,15-diene and 8a-hydroxy-ent-pimar-15-ene, and variable amounts of ent-kaur-16-ene
I638V
site-directed mutagenesis, the mutant shows unaltered product spectrum compared to the wild-type enzyme and produces just ent-kaur-16-ene
D75A
site-directed mutagenesis
D75C
site-directed mutagenesis, mutant crystal structure determination and analysis
D79C
site-directed mutagenesis
R204A
site-directed mutagenesis
D75A
Bradyrhizobium diazoefficiens JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110
-
site-directed mutagenesis
-
D75C
Bradyrhizobium diazoefficiens JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110
-
site-directed mutagenesis, mutant crystal structure determination and analysis
-
D79C
Bradyrhizobium diazoefficiens JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110
-
site-directed mutagenesis
-
R204A
Bradyrhizobium diazoefficiens JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110
-
site-directed mutagenesis
-
H212A
I645T
I664A
site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, the mutant produces significant amounts of 8alpha-hydroxy-ent-pimara-15-ene generated by addition of water prior to deprotonation, it also produces ent-copalol, the dephosphorylated derivative of ent-copalyl diphosphate
I664S
site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, the mutant produces significant amounts of 8alpha-hydroxy-ent-pimara-15-ene generated by addition of water prior to deprotonation, it also produces ent-copalol, the dephosphorylated derivative of ent-copalyl diphosphate
I664T
site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, the mutant produces high amounts of ent-pimara-8(14),15-diene, it also produces ent-copalol, the dephosphorylated derivative of ent-copalyl diphosphate
I664V
site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme, the mutant produces ent-isokaurene and ent-copalol, the dephosphorylated derivative of ent-copalyl diphosphate
D132A
low activity
D320A
low activity
D656A
no activity
A710C
no change in reaction product profile compared to wild-type
A710F
mutant converts geranylgeranyl diphosphate to ent-kaurene as the sole product
A710G
no change in reaction product profile compared to wild-type
A710M
mutant converted geranylgeranyl diphosphate to ent-kaurene as the sole product
A710N
no change in reaction product profile compared to wild-type
A710S
no change in reaction product profile compared to wild-type
D635A/D636A
mutation of the DDYFD motif in the kaurene synthase domain
H302A
I741T
I619A
site-directed mutagenesis, the mutant shows altered product spectrum compared to the wild-type enzyme
A676T
site-directed mutagenesis of isozyme RcKSL2, mutation of the catalytically important residue results in 60fold increased product amount composed of essentially the same mix of ent-trachylobane and ent-kaurene as observed for the wild-type enzyme
additional information