Cloned (Comment) | Organism |
---|---|
as a GST-fusion protein for expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
16-aza-ent-beyerane | - |
Arabidopsis thaliana | |
16-aza-ent-trachylobane | - |
Arabidopsis thaliana | |
ent-beyeran-16-yl diphosphate | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
[15-3H]ent-copalyl diphosphate | pseudo-binding constant for [15-3H]ent-copalyl diphosphate with GST-recombinant kaurene synthase | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | Arabidopsis thaliana | - |
ent-kaurene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SAK2 | chloroplast precursor | - |
Purification (Comment) | Organism |
---|---|
purified by affinity chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | - |
Arabidopsis thaliana | ent-kaurene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ent-kaurene synthase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
activity assay at room temperature | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
activity assay | Arabidopsis thaliana |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000004 | - |
in the presence of diphosphate | Arabidopsis thaliana | 16-aza-ent-beyerane | |
0.00002 | - |
in the presence of diphosphate | Arabidopsis thaliana | 16-aza-ent-trachylobane | |
0.0001 | - |
in the absence of diphosphate | Arabidopsis thaliana | 16-aza-ent-beyerane | |
0.001 | - |
in the absence of diphosphate | Arabidopsis thaliana | 16-aza-ent-trachylobane | |
0.01 | - |
in the absence of diphosphate | Arabidopsis thaliana | ent-beyeran-16-yl diphosphate |