Cloned (Comment) | Organism |
---|---|
in Escherichia coli as glutathione-S-transferase fusion protein | Phaeosphaeria sp. |
Protein Variants | Comment | Organism |
---|---|---|
D132A | low activity | Phaeosphaeria sp. |
D320A | low activity | Phaeosphaeria sp. |
D656A | no activity | Phaeosphaeria sp. |
additional information | several N- and C-terminal truncated enzymes produced, all found to be inactive | Phaeosphaeria sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | Phaeosphaeria sp. | involved in biosynthesis of gibberellins | ent-kaurene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaeosphaeria sp. | O13284 | - |
- |
Phaeosphaeria sp. L487 | O13284 | - |
- |
Purification (Comment) | Organism |
---|---|
from E. coli | Phaeosphaeria sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-copalyl diphosphate | - |
Phaeosphaeria sp. | ent-kaurene + diphosphate | - |
? | |
ent-copalyl diphosphate | involved in biosynthesis of gibberellins | Phaeosphaeria sp. | ent-kaurene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) | Phaeosphaeria sp. |