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Literature summary for 4.2.3.19 extracted from

  • Yamaguchi, S.; Saito, T.; Abe, H.; Yamane, H.; Murofushi, N.; Kamiya, Y.
    Molecular cloning and characterization of a cDNA encoding the gibberellin biosynthetic enzyme ent-kaurene synthase B from pumpkin (Cucurbita maxima L.) (1996), Plant J., 10, 203-213.
    View publication on PubMed
Search for more literature for 4.2.3.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as fusion protein with maltose-binding protein, enzyme exclusively shows EC 4.2.3.19 activity Cucurbita maxima

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Cucurbita maxima 9536
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
 SDS-PAGE, native enzyme Cucurbita maxima
89000
-
 molecular weight of native enzyme predicted from DNA sequence Cucurbita maxima
130000
-
 SDS-PAGE, cloned fusion protein Cucurbita maxima
131000
-
 molecular weight of cloned fusion protein predicted from DNA sequence Cucurbita maxima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Cucurbita maxima involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Cucurbita maxima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cloned enzyme from E. coli and native enzyme from endosperm Cucurbita maxima

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Cucurbita maxima
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
 Cucurbita maxima ent-kaurene + diphosphate
-
 ?
ent-copalyl diphosphate involved in biosynthesis of gibberellins Cucurbita maxima ent-kaurene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Cucurbita maxima