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Literature summary for 4.2.3.19 extracted from

  • Toyomasu, T.; Kawaide, H.; Ishizaki, A.; Shinoda, S.; Otsuka, M.; Mitsuhashi, W.; Sassa, T.
    Cloning of a full-length cDNA encoding ent-kaurene synthase from Gibberella fujikuroi: functional analysis of a bifunctional diterpene cyclase (2000), Biosci. Biotechnol. Biochem., 64, 660-664.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109, enzyme shows EC 4.2.3.19 and EC 5.5.1.13 activity Fusarium fujikuroi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
107000
-
predicted from cDNA sequence Fusarium fujikuroi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Fusarium fujikuroi involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Fusarium fujikuroi Q9UVY5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
Fusarium fujikuroi ent-kaurene + diphosphate
-
?
ent-copalyl diphosphate involved in biosynthesis of gibberellins Fusarium fujikuroi ent-kaurene + diphosphate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 107000 Fusarium fujikuroi

Synonyms

Synonyms Comment Organism
More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Fusarium fujikuroi