4.1.3.17: 4-hydroxy-4-methyl-2-oxoglutarate aldolase
This is an abbreviated version!
For detailed information about 4-hydroxy-4-methyl-2-oxoglutarate aldolase, go to the full flat file.
Reaction
Synonyms
4-hydroxy-4-methyl-2-ketoglutarate aldolase, 4-hydroxy-4-methyl-2-oxoglutarate aldolase, 4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase, aldolase, 4-hydroxy-4-methyl-2-oxoglutarate, gamma-methyl-gamma-hydroxy-alpha-ketoglutaric aldolase, HMG aldolase, HMG/CHA aldolase, KDPG aldolase, MHK aldolase, MHKG aldolase, pyruvate aldolase
ECTree
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Turnover Number
Turnover Number on EC 4.1.3.17 - 4-hydroxy-4-methyl-2-oxoglutarate aldolase
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0.058
-
wild type protein, 1.0 mM Mg2+, pH 8.0, 25°C
0.25
2-hydroxy-4-oxopentanedioic acid
-
wild type protein, 1.0 mM Mn2+, pH 8.0, 25°C
13.4
wild type enzyme, in the absence of phosphate, at pH 8.0 and 25°C
145
4-carboxy-4-hydroxy-2-oxoadipate
wild type enzyme, in the presence of 2 mM phosphate, at pH 8.0 and 25°C
0.048
-
wild type protein, 1.0 mM Mg2+, pH 8.0, 25°C
0.19
4-hydroxy-2-oxopentanoate
-
wild type protein, 1.0 mM Mn2+, pH 8.0, 25°C
0.014
mutant enzyme K147A, in the absence of phosphate, at pH 8.0 and 25°C
0.014
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme N71A, in the absence of phosphate, at pH 8.0 and 25°C
0.0184
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme N71A, in the presence of 2 mM phosphate, at pH 8.0 and 25°C
0.0224
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme K147A, in the presence of 2 mM phosphate, at pH 8.0 and 25°C
0.0276
4-hydroxy-4-methyl-2-oxoglutarate
-
pH 8.0, 25°C, RraA-like protein Yer010Cp
0.07
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme R123K, in the presence of 2 mM phosphate, at pH 8.0 and 25°C
0.086
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme R123K, in the absence of phosphate, at pH 8.0 and 25°C
0.099
4-hydroxy-4-methyl-2-oxoglutarate
-
R123K mutant protein, pH 8.0, 25°C
0.215
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme G144V, in the absence of phosphate, at pH 8.0 and 25°C
0.356
4-hydroxy-4-methyl-2-oxoglutarate
-
pH 8.0, 25°C, RraA-like protein TtRraA
0.394
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme G144V, in the presence of 2 mM phosphate, at pH 8.0 and 25°C
0.889
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme T145A, in the absence of phosphate, at pH 8.0 and 25°C
6.7
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme R40A, in the absence of phosphate, at pH 8.0 and 25°C
6.72
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme R195A, in the absence of phosphate, at pH 8.0 and 25°C
10.7
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme T145A, in the presence of 2 mM phosphate, at pH 8.0 and 25°C
11.5
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme R195A, in the presence of 2 mM phosphate, at pH 8.0 and 25°C
13.7
4-hydroxy-4-methyl-2-oxoglutarate
-
wild type protein, 1.0 mM Mn2+, pH 8.0, 25°C
15.6
4-hydroxy-4-methyl-2-oxoglutarate
wild type enzyme, in the absence of phosphate, at pH 8.0 and 25°C
19.3
4-hydroxy-4-methyl-2-oxoglutarate
-
wild type protein, 1.0 mM Mg2+, pH 8.0, 25°C
22.3
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme H75A, in the absence of phosphate, at pH 8.0 and 25°C
36.6
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme R40A, in the presence of 2 mM phosphate, at pH 8.0 and 25°C
53.5
4-hydroxy-4-methyl-2-oxoglutarate
mutant enzyme H75A, in the presence of 2 mM phosphate, at pH 8.0 and 25°C
134
4-hydroxy-4-methyl-2-oxoglutarate
wild type enzyme, in the presence of 2 mM phosphate, at pH 8.0 and 25°C
1.86
oxaloacetate
wild type enzyme, in the absence of phosphate, at pH 8.0 and 25°C
3.16
oxaloacetate
wild type enzyme, in the presence of 2 mM phosphate, at pH 8.0 and 25°C