Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.6.5.3: protein-synthesizing GTPase

This is an abbreviated version!
For detailed information about protein-synthesizing GTPase, go to the full flat file.

Word Map on EC 3.6.5.3

Reaction

GTP
+
H2O
=
GDP
 +
phosphate

Synonyms

50S ribosomal subunit assembly factor, aEF-1alpha, aIF2, aIF2 GTPase, aIF2-gamma, aIF2B, aIF2gamma, aIF5B, archaeal initiation factor 2, archaeal initiation factor 2C, archaeal translation initiation factor 2, BipA, BPI-inducible protein A, Bst-IF2, chloroplast elongation factor G, EC 3.6.1.48, Ec-L10, Ec-L11, Ec-L12, Eco-IF2, eEF1A, eEF2, EF 1 alpha, EF-1alpha, EF-1beta, EF-2, EF-4, EF-G, EF-G GTPase, EF-G1, EF-G1mt, EF-like GTPase, EF-Tu, EF-Tumt, EF4, EFL, Efl1, Efl1 GTPase, eIEF2, eIF 2, eIF2, eIF2A, eIF2alpha, eIF2B, eIF5, eIF5B, elongation factor, elongation factor (EF), elongation factor 1 alpha, elongation factor 1alpha, elongation factor 2, elongation factor 4, elongation factor G, elongation factor thermo unstable, elongation factor Tu, elongation factor-1alpha, elongation factor-1beta, elongation factor-2, elongation factor-like 1, elongation factor-like 1 GTPase, eukaryotic elongation factor 2, eukaryotic elongation factor one alpha, eukaryotic initiation factor 2, eukaryotic initiation factor 2A, eukaryotic initiation factor 5B, eukaryotic translation initiation factor 2, eukaryotic translation initiation factor 5B, fusA, GTP phosphohydrolase, GTPase, GTPase aIF5B, GTPase HflX, GTPase-activating protein, guanine triphosphatase, guanine-nucleotide-exchange factor of eIF2, guanosine 5'-triphosphatase, guanosine triphosphatase, HflX, HflX GTPase, IF-2, IF1, IF2, IF2 GTPase, IF2alpha, IF3, IF5B, infB, initiation factor (IF), initiation factor 2, initiation factor 3, initiation factor 5B, initiation factor aIF5B, initiation factor-2, L10, L11, L12, LepA, mitochondrial elongation factor G, mitochondrial initiation factor 2, peptide-release or termination factor, protein-synthesizing GTPase, protein-sythesizing GTPase, RF3, ribosomal GTPase, ribosome-bound initiation factor 2, ribosome-dependent GTPase, SelB, selenocysteine tRNA-specific elongation factor, signal recognition particle GTPase Ffh, SRP GTPase Ffh, Ss-aIF2, SsEF-1alpha, SsEF-2, SsGBP, SSO0269, SSO0412, SSO1050, SSO2381, SsoHflX, translation elongation factor 2, translation factor aIF2/5B, translation initiation factor, translation initiation factor 2, translation initiation factor 2 gamma, translation initiation factor 5B, translation initiation factor eIF5, translation initiation factor IF1, translation initiation factor IF2, translation termination factor eRF3, translational GTPase, translational guanosine triphosphatase, translational initiation factor 2, trGTPase, Tt-L11, ymIF2

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.3 protein-synthesizing GTPase

Purification

Purification on EC 3.6.5.3 - protein-synthesizing GTPase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE Sephadex A-50 column
-
purified as a complex with GDP
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta(DE3) by heat treatment at 80°C for 20 min, nickel affinity chromatography, dialysis, cation exchange chromatography, followed gel filtration, dialysis, and ultrafiltration
-
recombinant C2 subdomain of ymIF2
-
recombinant EF-G mutants 58C and 196C
-
recombinant enzyme from Escherichia coli by heat treatment at 65°C for 20 min, ultracentrifugation, hydrophobic interaction chromatography, gel filtration, and ultrafiltration
recombinant enzyme from Escherichia coli strain BL21(DE3) Star by hydrophobic interaction, anion exchange chromatography, gel filtration
recombinant GB1-His6-tagged wild-type eIF5B and truncated mutants eIF5B587-1220 (GH-DELTAeIF5B), eIF5B951-1220 comprising domains 3 and 4 (GH-eIF5B-D34), and eIF5B1076-1220 comprising domain 4 (GH-eIF5B-D4) by nickel affinity chromatography, tag cleavage by TEV protease, and anion exchange chromatography to remove the GB1 tag
O60841, P05198; P20042; P41091
recombinant His-tagged enzyme from Saccharomyces cerevisiae strain BCY123 by nickel affinity chromatography, tag cleavage by TEV protease, and another step of nickel affinity chromatography, followed by gel filtration
recombinant His-tagged IF2 from Escherichia coli by affinity chromatography and ion exchange chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chhrmatography and gel filtration
recombinant N-terminally His6-tagged enzyme EF4 from Escherichia coli strain BL21(DE3) Rosetta T1R by nickel affinity chromatography, cleavage of the His-tag, followed by gel filtration and anion exchange chromatography
recombinant wild-type and selenomethionine-labelled enzymes from Escherichia coli by hydrophobic interaction chromatography