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3.6.5.3: protein-synthesizing GTPase

This is an abbreviated version!
For detailed information about protein-synthesizing GTPase, go to the full flat file.

Word Map on EC 3.6.5.3

Reaction

GTP
+
H2O
=
GDP
 +
phosphate

Synonyms

50S ribosomal subunit assembly factor, aEF-1alpha, aIF2, aIF2 GTPase, aIF2-gamma, aIF2B, aIF2gamma, aIF5B, archaeal initiation factor 2, archaeal initiation factor 2C, archaeal translation initiation factor 2, BipA, BPI-inducible protein A, Bst-IF2, chloroplast elongation factor G, EC 3.6.1.48, Ec-L10, Ec-L11, Ec-L12, Eco-IF2, eEF1A, eEF2, EF 1 alpha, EF-1alpha, EF-1beta, EF-2, EF-4, EF-G, EF-G GTPase, EF-G1, EF-G1mt, EF-like GTPase, EF-Tu, EF-Tumt, EF4, EFL, Efl1, Efl1 GTPase, eIEF2, eIF 2, eIF2, eIF2A, eIF2alpha, eIF2B, eIF5, eIF5B, elongation factor, elongation factor (EF), elongation factor 1 alpha, elongation factor 1alpha, elongation factor 2, elongation factor 4, elongation factor G, elongation factor thermo unstable, elongation factor Tu, elongation factor-1alpha, elongation factor-1beta, elongation factor-2, elongation factor-like 1, elongation factor-like 1 GTPase, eukaryotic elongation factor 2, eukaryotic elongation factor one alpha, eukaryotic initiation factor 2, eukaryotic initiation factor 2A, eukaryotic initiation factor 5B, eukaryotic translation initiation factor 2, eukaryotic translation initiation factor 5B, fusA, GTP phosphohydrolase, GTPase, GTPase aIF5B, GTPase HflX, GTPase-activating protein, guanine triphosphatase, guanine-nucleotide-exchange factor of eIF2, guanosine 5'-triphosphatase, guanosine triphosphatase, HflX, HflX GTPase, IF-2, IF1, IF2, IF2 GTPase, IF2alpha, IF3, IF5B, infB, initiation factor (IF), initiation factor 2, initiation factor 3, initiation factor 5B, initiation factor aIF5B, initiation factor-2, L10, L11, L12, LepA, mitochondrial elongation factor G, mitochondrial initiation factor 2, peptide-release or termination factor, protein-synthesizing GTPase, protein-sythesizing GTPase, RF3, ribosomal GTPase, ribosome-bound initiation factor 2, ribosome-dependent GTPase, SelB, selenocysteine tRNA-specific elongation factor, signal recognition particle GTPase Ffh, SRP GTPase Ffh, Ss-aIF2, SsEF-1alpha, SsEF-2, SsGBP, SSO0269, SSO0412, SSO1050, SSO2381, SsoHflX, translation elongation factor 2, translation factor aIF2/5B, translation initiation factor, translation initiation factor 2, translation initiation factor 2 gamma, translation initiation factor 5B, translation initiation factor eIF5, translation initiation factor IF1, translation initiation factor IF2, translation termination factor eRF3, translational GTPase, translational guanosine triphosphatase, translational initiation factor 2, trGTPase, Tt-L11, ymIF2

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.3 protein-synthesizing GTPase

Cloned

Cloned on EC 3.6.5.3 - protein-synthesizing GTPase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. I15V. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a increased efficiency in protein synthesis, which is accompanied by an reduced affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an decreased efficiency in the intrinsic GTPase activity
comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. V15I. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a reduced efficiency in protein synthesis, which is accompanied by an increased affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an increased efficiency in the intrinsic GTPase activity
eEF2 in vitro translation by rabbit reticulocyte lysate
expressed in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli BL21
expression in Escherichia coli using the pT7-7 expression vector
-
for expression in Escherichia coli cells
-
full-length enzyme or C2 subdomain of ymIF2 expressed in Escherichia coli. The full-length ymIF2 can substitute for Escherichia coli IF2 in the formation of a functional initiation complex on 70S Escherichia coli ribosomes capable of forming the first peptide bond
-
gene EIF2B1, recombinant expression of C-terminally His6-tagged eIF2B subunit alpha in Escherichia coli strain Rosetta2(DE3)
P05198; P20042; P41091
gene fusA, recombinant expression of the N-terminal domain (74 residues) of Thermus thermophilus ribosomal protein L9 fused to the N-terminus of EF-G connecting residues 1-74 of ribosomal protein L9 and residues 8-691 of EF-G in Escherichia coli strain BL21(DE3) Star
gene inB, recombinant expression in Escherichia coli strain BL21infB::kanR, that has an inactivated endogenous infB gene
gene lepA, recombinant expression of N-terminally His6-tagged enzyme EF4 in Escherichia coli strain BL21(DE3) Rosetta T1R
gene lepA, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
high level expression of mutant enzyme P269S in Escherichia coli
-
His-tagged version of ymIF2 lacking its predicted mitochondrial presequence is expressed in Escherichia coli
-
in vitro translation of the enzyme using the PURE reconstituted translation system derived from Escherichia coli
mutant enzyme Q290L, expression in Escherichia coli
-
overexpression of all three subunits of human eIEF2 independently, and together in Sf9 cells using pFast Bac HT vector of baculovirus expression system. the expression of all subunits increases in infection time up to 72 h. Expression of the mutant forms S51A, S51D and S48A
-
recombinant expression in Escherichia coli strain BL21(DE3)
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta(DE3)
-
recombinant expression of EF-G mutants 58C and 196C
-
recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae strain BCY123
recombinant expression of His-tagged IF2 in Escherichia coli using the T5 expression system
recombinant expression of nontagged wild-type full-length enzyme in Escherichia coli strain BL21(DE3) and of selenomethionine-labelled enzyme in Escherichia coli strain B834 (DE3)
recombinant expression of wild-type eIF5B and truncated mutants eIF5B587-1220 (GH-DELTAeIF5B), eIF5B951-1220 comprising domains 3 and 4 (GH-eIF5B-D34), and eIF5B1076-1220 comprising domain 4 (GH-eIF5B-D4), all with an N-terminal GB1 tag, His6-tag, and a TEV protease cleavage site (GH-tag)
O60841, P05198; P20042; P41091
recombinant SsEF-1alpha and its nucleotide-free form are prepared by using an Escherichia coli expression system
-
the gene encoding SsEF-1alpha from the strain MT3 is cloned, sequenced and expressed in Escherichia coli, the vectors pGEM-3Z and pET22 are used
the SsEF-1alpha gene is cloned into the pT7-7 vector for expression in Escherichia coli BL21DE3 cells, in addition two truncated forms of SsEF-1alpha are constructed, encoding the domain G and the domains G+M
-