Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.5.3 extracted from

  • Hwang, Y.W.; Miller, D.L.
    A mutation that alters the nucleotide specificity of elongation factor Tu, a GTP regulatory protein (1987), J. Biol. Chem., 262, 13081-13085.
    View publication on PubMed
Search for more literature for 3.6.5.3

Application

Application Comment Organism
medicine polypeptide chain synthesis Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
D138N mutant with decreased affinity for GDP and increased affinity for XDP Escherichia coli

General Stability

General Stability Organism
EF-Tu(138N) not functional in vivo Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + H2O Escherichia coli
-
 ?
-
 ?
GDP + H2O Escherichia coli HW110
-
 ?
-
 ?
GTP + H2O Escherichia coli
-
 GDP + phosphate
-
 ?
GTP + H2O Escherichia coli HW110
-
 GDP + phosphate
-
 ?
ITP + H2O Escherichia coli
-
 IDP + phosphate
-
 ?
ITP + H2O Escherichia coli HW110
-
 IDP + phosphate
-
 ?
XDP + H2O Escherichia coli
-
 XMP + phosphate
-
 ?
XDP + H2O Escherichia coli HW110
-
 XMP + phosphate
-
 ?
XTP + H2O Escherichia coli
-
 XDP + phosphate
-
 ?
XTP + H2O Escherichia coli HW110
-
 XDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 and strains EF-Tu (wt) and EF-Tu (138N)
-
Escherichia coli
-
 elongations factor (EF)Tu
-
Escherichia coli HW110
-
 and strains EF-Tu (wt) and EF-Tu (138N)
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aurodox + H2O
-
 Escherichia coli ?
-
 ?
GDP + H2O
-
 Escherichia coli ?
-
 ?
GDP + H2O
-
 Escherichia coli HW110 ?
-
 ?
GTP + H2O
-
 Escherichia coli GDP + phosphate
-
 ?
GTP + H2O
-
 Escherichia coli HW110 GDP + phosphate
-
 ?
ITP + H2O
-
 Escherichia coli IDP + phosphate
-
 ?
ITP + H2O
-
 Escherichia coli HW110 IDP + phosphate
-
 ?
XDP + H2O
-
 Escherichia coli XMP + phosphate
-
 ?
XDP + H2O
-
 Escherichia coli HW110 XMP + phosphate
-
 ?
XTP + H2O
-
 Escherichia coli XDP + phosphate
-
 ?
XTP + H2O
-
 Escherichia coli HW110 XDP + phosphate
-
 ?