Literature summary for 3.6.5.3 extracted from

Masullo, M.; Cantiello, P.; Lamberti, A.; Longo, O.; Fiengo, A.; Arcari, P.
Cloning, expression and evolution of the gene encoding the elongation factor 1alpha from a low thermophilic Sulfolobus solfataricus strain (2003), FEMS Microbiol. Lett., 218, 285-290.

Search for more literature for 3.6.5.3

Cloned(Commentary)

Cloned (Comment)	Organism
comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. I15V. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a increased efficiency in protein synthesis, which is accompanied by an reduced affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an decreased efficiency in the intrinsic GTPase activity	Saccharolobus solfataricus
comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. V15I. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a reduced efficiency in protein synthesis, which is accompanied by an increased affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an increased efficiency in the intrinsic GTPase activity	Saccharolobus solfataricus
the gene encoding SsEF-1alpha from the strain MT3 is cloned, sequenced and expressed in Escherichia coli, the vectors pGEM-3Z and pET22 are used	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
additional information	comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. I15V. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a increased efficiency in protein synthesis, which is accompanied by an reduced affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an decreased efficiency in the intrinsic GTPase activity. The exchange has only very marginal effects on the thermal properties of the enzyme	Saccharolobus solfataricus
additional information	comparison of sequence with that of Sulfolobus solfataricus strain MT4 shows only one amino acid change, i.e. V15I. The difference is in the first guanine nucleotide binding consensus sequence G13HIDHGK and is responsible for a reduced efficiency in protein synthesis, which is accompanied by an increased affinity for both guanosine diphosphate (GDP) and guanosine triphosphate (GTP), and an increased efficiency in the intrinsic GTPase activity. The exchange has only very marginal effects on the thermal properties of the enzyme	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0009	-	GTP	SsMT4EF-1alpha	Saccharolobus solfataricus
0.0027	-	GTP	SsMT3EF-1alpha	Saccharolobus solfataricus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
ribosome	-	Saccharolobus solfataricus	5840	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Saccharolobus solfataricus	-	GDP + phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	elongation factor 1alpha from strain MT3 with optimum growth temperature 75°C	-
Saccharolobus solfataricus	-	elongation factor 1alpha from strain MT4 with optimum growth temperature 87°C	-
Saccharolobus solfataricus	P35021	strain MT3, optimal growth temperature 75°C, strain MT4, optimal growth temperature 87°C	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Saccharolobus solfataricus	GDP + phosphate	-	ir

Synonyms

Synonyms	Comment	Organism
EF-1alpha	-	Saccharolobus solfataricus
elongation factor 1alpha	-	Saccharolobus solfataricus
protein-sythesizing GTPase	-	Saccharolobus solfataricus
SsEF-1alpha	amino acid at position 15, strain MT3 valine, SsMT3EF-1alpha, strain MT4 isoleucine, SsMT4EF-1alpha	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
87	-	SsMT3EF-1alpha	Saccharolobus solfataricus
91	-	SsMT4EF-1alpha	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.013	-	GTP	SsMT3EF-1alpha	Saccharolobus solfataricus
0.015	-	GTP	SsMT4EF-1alpha	Saccharolobus solfataricus

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Release 2023.1 (January 2023)