EC Number   |
|---|
   3.6.5.3 | - |
| 3.6.5.3 | DEAE Sephadex A-50 column |
| 3.6.5.3 | purified as a complex with GDP |
| 3.6.5.3 | recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta(DE3) by heat treatment at 80°C for 20 min, nickel affinity chromatography, dialysis, cation exchange chromatography, followed gel filtration, dialysis, and ultrafiltration |
| 3.6.5.3 | recombinant C2 subdomain of ymIF2 |
| 3.6.5.3 | recombinant EF-G mutants 58C and 196C |
| 3.6.5.3 | recombinant enzyme from Escherichia coli by heat treatment at 65°C for 20 min, ultracentrifugation, hydrophobic interaction chromatography, gel filtration, and ultrafiltration |
| 3.6.5.3 | recombinant enzyme from Escherichia coli strain BL21(DE3) Star by hydrophobic interaction, anion exchange chromatography, gel filtration |
| 3.6.5.3 | recombinant GB1-His6-tagged wild-type eIF5B and truncated mutants eIF5B587-1220 (GH-DELTAeIF5B), eIF5B951-1220 comprising domains 3 and 4 (GH-eIF5B-D34), and eIF5B1076-1220 comprising domain 4 (GH-eIF5B-D4) by nickel affinity chromatography, tag cleavage by TEV protease, and anion exchange chromatography to remove the GB1 tag |
| 3.6.5.3 | recombinant His-tagged enzyme from Saccharomyces cerevisiae strain BCY123 by nickel affinity chromatography, tag cleavage by TEV protease, and another step of nickel affinity chromatography, followed by gel filtration |
