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3.6.1.5: apyrase

This is an abbreviated version!
For detailed information about apyrase, go to the full flat file.

Word Map on EC 3.6.1.5

Reaction

a nucleoside 5'-diphosphate
+
H2O
=
a nucleoside 5'-phosphate
 +
phosphate

Synonyms

5' ectonucleotidase, 5'-nucleotidase, adenosine diphosphatase, adenosine pyrophosphatase, adenylpyrophophatase, ADPase, APT102, Apy, Apy 1, Apy 2, APY-1, APY1, APY10, APY2, APY2-type apyrase, APY4, APY5, APY6, APY7, APY8, APY9, Apyrase, apyrase 2, APYRASE2, At1g14230, At1g14240, At1g14250, At2g02970, At3g04080, At5g18280, AtAPY1, AtAPY2, AtAPY3, AtAPY4, AtAPY5, AtAPY6, ATP diphosphohydrolase, ATP diphosphohyrolase, ATP-diphosphatase, ATP-diphosphohydrolase, ATP-diphosphohydrolase 2, ATPase, ATPase 2, ATPDase, ATPDase1, ATPDase2, BjAPY2, Ca2+-activated nucleoside diphosphatase, CApy, CD39, CD39 antigen, CD39-like ATP diphosphohydrolase, CD39L1, Def11, E-NTPDase, E-NTPDase 3, ecto-5'-nucleotidase, ecto-apyrase, ecto-ATP diphosphohydrolase, ecto-ATP-diphosphohydrolase, ecto-ATPase, ecto-ATPDase, ecto-NTPDase, Ecto-NTPDase1, ecto-nucleoside triphosphate diphosphohydrolase, ecto-nucleoside triphosphate diphosphohydrolase 1, ecto-nucleoside triphosphate diphosphohydrolase 2, ecto-nucleoside triphosphate diphosphohydrolase1, ecto-nucleoside triphosphate diphosphohydrolase2, ecto-nucleoside triphosphate diphosphohydrolase3, ecto-nucleoside triphosphate diphosphohydrolase8, ecto-nucleoside triphosphate-diphosphohydrolase, ecto-nucleoside-triphosphate-diphosphohydrolase, ectoapyrase, ectonucleoside triphosphate diphosphohydrolase, ectonucleoside triphosphate diphosphohydrolase 3, ectonucleotidase, ENTPD1, ENTPD2, ENTPD3, Entpd8, ENTPDase1, esctonucleoside triphosphate diphosphohydrolase 1, esctonucleoside triphosphate diphosphohydrolase 2, esctonucleoside triphosphate diphosphohydrolase 8, Golgi nucleoside diphosphatase, GS50, GS52, HB6, hCD39, LALP1, LBRM_15_0030, LicNTPDase-2, LINJ_15_0030, LmjF15.0030, LmNTPDase 1, Lpg1905, lumenal NDPase, Lymphoid cell activation antigen, More, MP67, MpAPY2, NTPase, NTPD1, NTPDase, NTPDase 1, NTPDase 2, NTPDase 3, NTPDase 5, NTPDase 6, NTPDase 8, NTPDase1, NTPDase1/CD39, NTPDase2, NTPDase2/CD39L1, NTPDase3, NTPDase8, nucleoside triphosphatase, nucleoside triphosphate diphosphohydrolase, nucleoside triphosphate diphosphohydrolase 1, nucleoside triphosphate diphosphohydrolase 2, nucleoside triphosphate diphosphohydrolase 8, nucleoside triphosphate diphosphohydrolase-1, NDPDase 1, nucleoside triphosphate diphosphohydrolase-2, nucleoside triphosphate diphosphohydrolase-3, nucleoside triphosphate diphosphohydrolase-8, nucleoside triphosphate disphophohydrolase, nucleoside triphosphate-diphosphohydrolase, nucleotide triphosphate diphosphohydrolase, PeAPY2, potato-specific apyrase, PsAPY1, psNTP9, r-NTPDase 1, r-NTPDase 2, r-NTPDase 3, r-NTPDase 8, Ruviapyrase, SCAN-1, snake venom apyrase, SolCD39/NTPDase1, soluble apyrase, soluble calcium-activated nucleotidase 1, soluble CD39, TaAPY1, TaAPY2, TaAPY3-1, TaAPY3-2, TaAPY3-3, TaAPY3-4, TaAPY5, TaAPY6, TaAPY7, XAPY

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.5 apyrase

Purification

Purification on EC 3.6.1.5 - apyrase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
anion exchange column chromatography and gel filtration
His SpinTrap column chromatography
His-Select nickel affinity column chromatography
HisTrap column chromatography, HiTrap Q column chromatography, and HiLoad S200 gel filtration
-
isoform APY6, Superdex 75 gel filtration
-
native enzyme 1.6fold (ATPase) and 2.3fold (ADPase) from venom by gel filtration and anion exchange chromatography
-
native enzyme 4915fold from brain by ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, anion exchange and hydroxyapatite chromatography, again gel filtration, and adsorption chromatography
-
native enzyme from salivary gland by anion exchange chromatography, ultrafiltration, and cation exchange chromatography
native enzyme partially by apoplast preparation
-
native enzyme to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and concanavalin A affinity chromatography, recombinant enzyme from Escherichia coli
partially
purified from liver
-
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant enzyme expressed in Pichia pastoris
-
recombinant enzyme from insect Sf9 cells by anion exchange chromatography to homogeneity, large scale preparation
-
recombinant GS52 from Escherichia coli and tabacco
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, denaturing by urea and renaturing using glutathione and dialysis
recombinant His-tagged soluble NTPDase2 extracellular domain from HEK-293 cells by ammonium sulfate fractionation, ultrafiltration, nickel affinity chromatography, and gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli
-
recombinant His-tagged wild-type and mutant NTPDase8s from HEK293 cell plasma membranes by ammonium sulfate fractionation and nickel affinity column chromatography
recombinant His6-tagged Lpg1905 from Escherichia coli BL21(DE3) C41 strain by nickel affinity chromatography
-
recombinant LicNTPDase-2
recombinant rCApy C-terminally fused to a His6/S-tag solubilized from inclusion bodies after expression in Escherichia coli strain BL21(DE3), by nickel and glutathione affinity chromatography
reconstitution of enzyme in lipid vesicles is associated with a decrease in KM value of nearly an order of magnitude over the detergent-solubilized form, with a concomitant increase in both ADPase and ATPase catalytic efficiencies
-
salivary enzyme, purification gives five N-glycosylated proteins of 88, 82, 79, 68, and 67 kDa molecular masses
two ATPDase isoforms
-