Information on EC 3.6.1.5 - apyrase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.6.1.5
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RECOMMENDED NAME
GeneOntology No.
apyrase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a nucleoside 5'-diphosphate + H2O = a nucleoside 5'-phosphate + phosphate
show the reaction diagram
(1b)
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a nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate
show the reaction diagram
overall reaction
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a nucleoside 5'-triphosphate + H2O = a nucleoside 5'-diphosphate + phosphate
show the reaction diagram
(1a)
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
NIL
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non-pathway related
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Purine metabolism
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Pyrimidine metabolism
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UTP and CTP dephosphorylation I
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UTP and CTP dephosphorylation II
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SYSTEMATIC NAME
IUBMB Comments
nucleoside triphosphate phosphohydrolase (nucleoside monophosphoate-forming)
Apyrases are active against both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyse NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates. They differ from ATPases, which specifically hydrolyse ATP, by hydrolysing both ATP and ADP. The eukaryotic enzymes requires Ca2+, but Mg2+ can substitute. Most of the ecto-ATPases that occur on the cell surface and hydrolyse extracellular nucleotides belong to this enzyme family.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-95-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
; recombinant enzyme
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
dog
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
chick-pea
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
gene capy
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
enzymes NT5E-1 and NT5E-2 encoded by genes CG4827 and CG30104
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Manually annotated by BRENDA team
variant TM-1
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Manually annotated by BRENDA team
barley
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Manually annotated by BRENDA team
Leishmania braziliensis MHOM/BR/1975/M2903
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Manually annotated by BRENDA team
mouse
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Manually annotated by BRENDA team
Orchopeas howardi
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Manually annotated by BRENDA team
Oropsylla bacchi
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Manually annotated by BRENDA team
rye
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Manually annotated by BRENDA team
strain RIA 57
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Manually annotated by BRENDA team
strain RIA 57
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Manually annotated by BRENDA team
cysticerci
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
Triticosecale Wittmack
triticale
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Manually annotated by BRENDA team
wheat
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
expression during all developmental stages tested
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
influence of transmembrane helix dynamics on activity is achieved by coupling to a domain motion. Active site structure of NTPDase1, overview, closure movement in NTPDases
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,N6-etheno-ADP + H2O
1,N6-etheno-AMP + phosphate
show the reaction diagram
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-
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?
1,N6-etheno-ATP + H2O
1,N6-etheno-AMP + phosphate
show the reaction diagram
-
-
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?
2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate + H2O
2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-phosphate + phosphate
show the reaction diagram
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-
-
-
?
2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate + H2O
2'(3')-O-(2,4,6-trinitrophenyl)adenosine 5'-phosphate + phosphate
show the reaction diagram
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-
-
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?
3'(2')-O-(methylanthranoyl)adenosine 5'-diphosphate + H2O
3'(2')-O-(methylanthranoyl)adenosine 5'-phosphate + phosphate
show the reaction diagram
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-
-
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?
3'(2')-O-(methylanthranoyl)adenosine 5'-triphosphate + H2O
3'(2')-O-(methylanthranoyl)adenosine 5'-phosphate + phosphate
show the reaction diagram
-
-
-
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?
4-nitrophenyl thymidine 5'-phosphate + H2O
4-nitrophenol + TMP
show the reaction diagram
-
-
-
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?
4-nitrophenylphosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
low activity
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?
5'-AMP + H2O
adenosine + phosphate
show the reaction diagram
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23% of activity compared to ATP
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?
8-oxo-dGTP + 2 H2O
8-oxo-dGMP + 2 phosphate
show the reaction diagram
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?
adenosine (5')-tetraphospho-(5')-adenosine + H2O
?
show the reaction diagram
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?
adenosine 5'-tetraphosphate + H2O
adenosine 5'-monophosphate + phosphate
show the reaction diagram
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?
adenosine 5'-[(alpha,beta)-methyleno] triphosphate + H2O
?
show the reaction diagram
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?
ADP + 2 H2O
adenosine + 2 phosphate
show the reaction diagram
ADP + H2O
?
show the reaction diagram
AB600992, AB600997
isoform MP67 demonstrates substantially higher substrate specificity for ADP than for ATP
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?
ADP + H2O
AMP + phosphate
show the reaction diagram
ADP-ribose + H2O
?
show the reaction diagram
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-
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?
AMP + H2O
adenosine + phosphate
show the reaction diagram
AMPCPP + H2O
?
show the reaction diagram
low activity
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?
ATP + 2 H2O
AMP + 2 phosphate
show the reaction diagram
ATP + H2O
ADP + phosphate
show the reaction diagram
ATP + H2O
AMP + 2 phosphate
show the reaction diagram
ATP + H2O
AMP + phosphate + H+
show the reaction diagram
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-
-
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?
CDP + H2O
CMP + phosphate
show the reaction diagram
CDP-choline + H2O
?
show the reaction diagram
-
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?
CTP + 2 H2O
CMP + 2 phosphate
show the reaction diagram
CTP + H2O
CDP + phosphate
show the reaction diagram
CTP + H2O
CMP + phosphate + H+
show the reaction diagram
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?
dATP + 2 H2O
dAMP + 2 phosphate
show the reaction diagram
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?
dATP + H2O
dAMP + phosphate
show the reaction diagram
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?
dATP + H2O
dAMP + phosphate + H+
show the reaction diagram
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?
dCTP + 2 H2O
dCMP + 2 phosphate
show the reaction diagram
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?
dCTP + H2O
dCMP + phosphate + H+
show the reaction diagram
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?
dGTP + 2 H2O
dGMP + 2 phosphate
show the reaction diagram
-
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?
dGTP + H2O
dGMP + phosphate + H+
show the reaction diagram
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?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
low activity
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?
dTTP + H2O
dTMP + phosphate
show the reaction diagram
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?
dTTP + H2O
dTMP + phosphate + H+
show the reaction diagram
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?
dUTP + H2O
dUMP + phosphate + H+
show the reaction diagram
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?
FAD + H2O
?
show the reaction diagram
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?
GDP + H2O
?
show the reaction diagram
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?
GDP + H2O
GDP + phosphate
show the reaction diagram
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35% of the activity with ATP
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?
GDP + H2O
GMP + phosphate
show the reaction diagram
GDP-mannose + H2O
?
show the reaction diagram
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?
GTP + 2 H2O
GMP + 2 phosphate
show the reaction diagram
GTP + H2O
GDP + phosphate
show the reaction diagram
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?
GTP + H2O
GMP + phosphate + H+
show the reaction diagram
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-
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?
IDP + H2O
?
show the reaction diagram
-
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?
IDP + H2O
IMP + phosphate
show the reaction diagram
ITP + 2 H2O
IMP + 2 phosphate
show the reaction diagram
ITP + H2O
IDP + phosphate
show the reaction diagram
ITP + H2O
IMP + phosphate
show the reaction diagram
NADH + H2O
?
show the reaction diagram
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?
oligophosphates + H2O
?
show the reaction diagram
-
n = 3, 15, 40, 60
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?
phosphoenolpyruvate + H2O
pyruvate + phosphate
show the reaction diagram
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?
TDP + H2O
TMP + phosphate
show the reaction diagram
tetraamine(imidodiphosphato)cobalt + H2O
?
show the reaction diagram
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?
thiamine diphosphate + 2 H2O
thiamine + 2 phosphate
show the reaction diagram
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?
thio-dATP + H2O
thio-dAMP + phosphate
show the reaction diagram
-
-
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?
TTP + 2 H2O
TMP + 2 phosphate
show the reaction diagram
TTP + H2O
TDP + phosphate
show the reaction diagram
-
low activity
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?
UDP + 2 H2O
uridine + 2 phosphate
show the reaction diagram
UDP + H2O
?
show the reaction diagram
best substrate
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?
UDP + H2O
UMP + phosphate
show the reaction diagram
UDP-glucose + H2O
?
show the reaction diagram
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?
UTP + 2 H2O
UMP + 2 phosphate
show the reaction diagram
UTP + H2O
UDP + phosphate
show the reaction diagram
UTP + H2O
UMP + phosphate
show the reaction diagram
UTP + H2O
UMP + phosphate + H+
show the reaction diagram
-
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + 2 H2O
adenosine + 2 phosphate
show the reaction diagram
ADP + H2O
AMP + phosphate
show the reaction diagram
AMP + H2O
adenosine + phosphate
show the reaction diagram
ATP + 2 H2O
AMP + 2 phosphate
show the reaction diagram
ATP + H2O
ADP + phosphate
show the reaction diagram
ATP + H2O
AMP + 2 phosphate
show the reaction diagram
CDP + H2O
CMP + phosphate
show the reaction diagram
CTP + 2 H2O
CMP + 2 phosphate
show the reaction diagram
GDP + H2O
GMP + phosphate
show the reaction diagram
GTP + 2 H2O
GMP + 2 phosphate
show the reaction diagram
TTP + 2 H2O
TMP + 2 phosphate
show the reaction diagram
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?
UDP + 2 H2O
uridine + 2 phosphate
show the reaction diagram
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?
UDP + H2O
UMP + phosphate
show the reaction diagram
UTP + 2 H2O
UMP + 2 phosphate
show the reaction diagram
-
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?
UTP + H2O
UMP + phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
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