Application | Comment | Organism |
---|---|---|
medicine | its unique enzymatic activity makes the salivary apyrase an attractive candidate as a therapeutic agent for the treatment of thrombotic pathologies | Phlebotomus duboscqi |
Cloned (Comment) | Organism |
---|---|
expressed as a thioredoxin-His-tagged fusion protein in Escherichia coli BL21 cells | Phlebotomus duboscqi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Phlebotomus duboscqi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | strictly dependent on Ca2+ | Phlebotomus duboscqi | |
additional information | not dependent on Mg2+ | Phlebotomus duboscqi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35200 | - |
mature isoform Apy 2, calculated from amino acid sequence | Phlebotomus duboscqi |
35800 | - |
mature isoform Apy 1, calculated from amino acid sequence | Phlebotomus duboscqi |
50000 | - |
recombinant Apy 2 thioredoxin-His-tagged fusion protein, SDS-PAGE | Phlebotomus duboscqi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phlebotomus duboscqi | Q06K73 | - |
- |
Phlebotomus duboscqi | Q06K77 | - |
- |
Purification (Comment) | Organism |
---|---|
His SpinTrap column chromatography | Phlebotomus duboscqi |
Renatured (Comment) | Organism |
---|---|
using 8 M urea, 50 mM Tris-HCl (pH 8.0), 1 mM EDTA and 1 mM dithiothreitol | Phlebotomus duboscqi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
salivary gland | - |
Phlebotomus duboscqi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Phlebotomus duboscqi | AMP + phosphate | - |
? | |
ATP + 2 H2O | - |
Phlebotomus duboscqi | AMP + 2 phosphate | - |
? | |
additional information | the enzyme does not hydrolyze AMP, GDP, CDP or UDP | Phlebotomus duboscqi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Apy 1 | isoform | Phlebotomus duboscqi |
Apy 2 | isoform | Phlebotomus duboscqi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Phlebotomus duboscqi | isoform Apy 1, calculated from amino acid sequence | - |
7.4 |
Phlebotomus duboscqi | isoform Apy 2, calculated from amino acid sequence | - |
9 |
General Information | Comment | Organism |
---|---|---|
physiological function | the recombinant protein inhibits ADP- and collagen-induced platelet aggregation. Thus, this salivary protein plays an important role in the blood-feeding process in Phlebotomus duboscqi | Phlebotomus duboscqi |