Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaF | 1 mM, inhibition | Triatoma infestans | |
vanadate | 1 mM, inhibition | Triatoma infestans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required, may be substituted by Mg2+, Mg2+ | Triatoma infestans | |
Mg2+ | required, may be substituted by Mn2+, Co2+ | Triatoma infestans | |
Mn2+ | required, may be substituted by Mg2+, Co2+ | Triatoma infestans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59800 | - |
x * 79000, glycosylated form, SDS-PAGE, x * 59800, unglycosylated mature form, calculated | Triatoma infestans |
79000 | - |
x * 79000, glycosylated form, SDS-PAGE, x * 59800, unglycosylated mature form, calculated | Triatoma infestans |
88000 | - |
x * 88000, 82000, 79000, 68000, or 67000, SDS-PAGE | Triatoma infestans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Triatoma infestans | enzyme inhibits ADP-induced human platelet aggregation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triatoma infestans | - |
five N-glycosylated isoforms of 88, 82, 79,68, and 67 kDa molecular masses | - |
Triatoma infestans | Q70GK8 | precursor molecule, 79 kDa isoform | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylated protein | Triatoma infestans |
glycoprotein | purification gives five N-glycosylated proteins of 88, 82, 79, 68, and 67 kDa molecular masses | Triatoma infestans |
proteolytic modification | sequence contains a 23 amino acid signal peptide | Triatoma infestans |
Purification (Comment) | Organism |
---|---|
salivary enzyme, purification gives five N-glycosylated proteins of 88, 82, 79, 68, and 67 kDa molecular masses | Triatoma infestans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
saliva | - |
Triatoma infestans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1200 | - |
hydrolysis of ADP, 37°C, pH 8.0 | Triatoma infestans |
1500 | - |
hydrolysis of ATP, 37°C, pH 8.0 | Triatoma infestans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | activity in decreasing order: ADP, IDP, CDP, GDP | Triatoma infestans | AMP + phosphate | - |
? | |
ATP + 2 H2O | activity in decreasing order: ATP, CTP, GTP, UTP, ITP | Triatoma infestans | AMP + 2 phosphate | - |
? | |
CDP + H2O | activity in decreasing order: ADP, IDP, CDP, GDP | Triatoma infestans | CMP + phosphate | - |
? | |
CTP + 2 H2O | activity in decreasing order: ATP, CTP, GTP, UTP, ITP | Triatoma infestans | CMP + 2 phosphate | - |
? | |
GDP + H2O | activity in decreasing order: ADP, IDP, CDP, GDP | Triatoma infestans | GMP + phosphate | - |
? | |
GTP + 2 H2O | activity in decreasing order: ATP, CTP, GTP, UTP, ITP | Triatoma infestans | GMP + 2 phosphate | - |
? | |
IDP + H2O | activity in decreasing order: ADP, IDP, CDP, GDP | Triatoma infestans | IMP + phosphate | - |
? | |
ITP + 2 H2O | activity in decreasing order: ATP, CTP, GTP, UTP, ITP | Triatoma infestans | IMP + 2 phosphate | - |
? | |
additional information | enzyme inhibits ADP-induced human platelet aggregation | Triatoma infestans | ? | - |
? | |
additional information | no substrate: nucleoside 5-monophosphates, glycerol phosphate, glycose 6-phosphate, UDP-galactose | Triatoma infestans | ? | - |
? | |
UTP + 2 H2O | activity in decreasing order: ATP, CTP, GTP, UTP, ITP | Triatoma infestans | UMP + 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, glycosylated form, SDS-PAGE, x * 59800, unglycosylated mature form, calculated | Triatoma infestans |
? | x * 88000, 82000, 79000, 68000, or 67000, SDS-PAGE | Triatoma infestans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
both hydrolysis of ATP and ADP | Triatoma infestans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
both hydrolysis of ATP and ADP | Triatoma infestans |