Cloned (Comment) | Organism |
---|---|
isoforms APY4, APY5 and APY6 are expressed in Escherichia coli BL21(DE3) cells with thioredoxin-tag, His6-tag and S-tag | Solanum tuberosum |
General Stability | Organism |
---|---|
the removal of thioredoxin-tag from purified apyrase causes 10times reduction of specific activity | Solanum tuberosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Solanum tuberosum | |
EDTA | complete inhibition at 10 mM | Solanum tuberosum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | best activator of isoform APY6 | Solanum tuberosum | |
Co2+ | activator of isoform APY6 | Solanum tuberosum | |
Mg2+ | an increase in ADPase activity in presence of Mg2+ ions occurs in case of isoform APY5 | Solanum tuberosum | |
Mn2+ | activator of isoform APY6 | Solanum tuberosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36989 | - |
x * 36989, isoform APY6, calculated from amino acid sequence | Solanum tuberosum |
48748 | - |
x * 48748, isoform APY5, calculated from amino acid sequence | Solanum tuberosum |
49765 | - |
x * 49765, isoform APY4, calculated from amino acid sequence | Solanum tuberosum |
50004 | - |
x * 50004, isoform APY9, calculated from amino acid sequence | Solanum tuberosum |
50032 | - |
x * 50032, isoform APY8, calculated from amino acid sequence | Solanum tuberosum |
50062 | - |
x * 50062, isoform APY10, calculated from amino acid sequence | Solanum tuberosum |
50077 | - |
x * 50077, isoform APY7, calculated from amino acid sequence | Solanum tuberosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | Solanum tuberosum | 68% activity compared to ATP | AMP + phosphate | - |
? | |
ATP + 2 H2O | Solanum tuberosum | 100% activity | AMP + 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | - |
variety Saturna | - |
Purification (Comment) | Organism |
---|---|
isoform APY6, Superdex 75 gel filtration | Solanum tuberosum |
Renatured (Comment) | Organism |
---|---|
isoform APY6 in 50 mM Tris-HCl pH 6.5 buffer containing 6 M guanidine hydrochloride and 10 mM 2-mercaptoethanol | Solanum tuberosum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tuber | - |
Solanum tuberosum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | 68% activity compared to ATP | Solanum tuberosum | AMP + phosphate | - |
? | |
ATP + 2 H2O | 100% activity | Solanum tuberosum | AMP + 2 phosphate | - |
? | |
CDP + H2O | 3.34% activity compared to ATP | Solanum tuberosum | CMP + phosphate | - |
? | |
CTP + 2 H2O | 12.5% activity compared to ATP | Solanum tuberosum | CMP + 2 phosphate | - |
? | |
GDP + H2O | 69.2% activity compared to ATP | Solanum tuberosum | GMP + phosphate | - |
? | |
GTP + 2 H2O | 84.6% activity compared to ATP | Solanum tuberosum | GMP + 2 phosphate | - |
? | |
UDP + H2O | 6.8% activity compared to ATP | Solanum tuberosum | UMP + phosphate | - |
? | |
UTP + 2 H2O | 28.2% activity compared to ATP | Solanum tuberosum | UMP + 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36989, isoform APY6, calculated from amino acid sequence | Solanum tuberosum |
? | x * 48748, isoform APY5, calculated from amino acid sequence | Solanum tuberosum |
? | x * 49765, isoform APY4, calculated from amino acid sequence | Solanum tuberosum |
? | x * 50004, isoform APY9, calculated from amino acid sequence | Solanum tuberosum |
? | x * 50032, isoform APY8, calculated from amino acid sequence | Solanum tuberosum |
? | x * 50062, isoform APY10, calculated from amino acid sequence | Solanum tuberosum |
? | x * 50077, isoform APY7, calculated from amino acid sequence | Solanum tuberosum |
Synonyms | Comment | Organism |
---|---|---|
APY10 | isoform | Solanum tuberosum |
APY4 | isoform | Solanum tuberosum |
APY5 | isoform | Solanum tuberosum |
APY6 | isoform | Solanum tuberosum |
APY7 | isoform | Solanum tuberosum |
APY8 | isoform | Solanum tuberosum |
APY9 | isoform | Solanum tuberosum |
NTPDase | - |
Solanum tuberosum |
nucleoside triphosphate diphosphohydrolase | - |
Solanum tuberosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
isoform APY6 | Solanum tuberosum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Solanum tuberosum | isoform APY4, calculated from amino acid sequence | - |
6.6 |
Solanum tuberosum | isoform APY7, calculated from amino acid sequence | - |
6.6 |
Solanum tuberosum | isoform APY8, calculated from amino acid sequence | - |
6.6 |
Solanum tuberosum | isoform APY9, calculated from amino acid sequence | - |
6.6 |
Solanum tuberosum | isoform APY10, calculated from amino acid sequence | - |
7.2 |
Solanum tuberosum | isoform APY5, calculated from amino acid sequence | - |
7.2 |
Solanum tuberosum | isoform APY6, calculated from amino acid sequence | - |
8.4 |