3.5.1.3: omega-amidase
This is an abbreviated version!
For detailed information about omega-amidase, go to the full flat file.
Word Map on EC 3.5.1.3
-
3.5.1.3
-
alpha-ketoglutaramate
-
asparagine
-
transamination
-
ammonia
-
oxaloacetate
-
succinamate
-
glutaminase
-
transamidase
-
analysis
-
medicine
-
biotechnology
- 3.5.1.3
- alpha-ketoglutaramate
- asparagine
-
transamination
- ammonia
- oxaloacetate
- succinamate
- glutaminase
-
transamidase
- analysis
- medicine
- biotechnology
Reaction
Synonyms
alpha-keto acid omega-amidase, alpha-keto acid-omega-amidase, amidase, omega, dicarboxylate omega-amidase, NIT2, Nit2/omega-amidase, nitrilase-like protein 2, omega-amidase, omega-amidase/Nit2, omega-amido dicarboxylate amidohydrolase, omega-amidodicarboxylate amidohydrolase
ECTree
3.5.1.3 omega-amidaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.5.1.3 - omega-amidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
butyl glutarate + hydoxylamine
glutaramate + butanol
-
hydroxaminolysis
-
-
?
ethyl glutarate + hydoxylamine
glutaramate + ethanol
-
hydroxaminolysis
-
-
?
ethyl succinate + hydoxylamine
succinamate + ethanol
-
hydroxaminolysis
-
-
?
fumarate + hydroxylamine
fumaryl hydroxamate + H2O
-
acyl transfer reaction
-
-
?
gamma-monomethyl-2-oxoglutaramate + H2O
gamma-monomethyl-2-oxoglutarate + NH3
-
tested at pH 7.2 and pH 8.5
-
-
?
glutaramate + methanol
glutarate methyl ester + hydroxylamine
-
methanolysis
-
-
?
L-asparagine + hydroxylamine
L-asparaginyl hydroxamate + H2O
-
amide transfer reaction
-
-
?
L-phenylalanine amide + H2O
L-Phe + NH3
-
poor substrate, 12% compared with alpha-ketoglutaramate
-
-
?
methyl succinate + hydoxylamine
succinamate + methanol
-
hydroxaminolysis
-
-
?
p-methylphenyl glutarate + hydroxylamine
glutaramate + 4-methylphenol
-
-
-
-
?
propyl glutarate + hydoxylamine
glutaramate + propanol
-
hydroxaminolysis
-
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
the product of glutamine transamination, 2-oxoglutaramate, can be hydrolyzed by the enzyme omega-amidase to form and ammonia
-
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
the product of glutamine transamination, 2-oxoglutaramate, can be hydrolyzed by the enzyme omega-amidase to form and ammonia
-
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
physiological function of omega-amidase might be hydrolysis or transfer of amide group of alpha-ketoglutarate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
the in vivo substrates are generated by transamination of glutamine and asparagine, respectively
-
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
the conserved Cys-Glu-Lys triad is involved in catalysis
-
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
sole substrate is the open-chain form of alpha-ketoglutaramate, not the cyclic form
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
the in vivo substrates are generated by transamination of glutamine and asparagine, respectively
-
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
enzyme activity measured in a 96-well plate format
-
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants
-
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
alpha-ketoglutaramate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
-
physiological function of omega-amidase might be hydrolysis or transfer of amide group of alpha-ketoglutarate
-
?
2-oxoglutaramate + H2O
2-oxoglutarate + NH3
glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants
-
-
?
2-oxoglutaramate + hydroxylamine
2-oxoglutaryl hydroxamate + NH3
-
alpha-ketoglutaramate
-
-
?
2-oxoglutaramate + hydroxylamine
2-oxoglutaryl hydroxamate + NH3
-
no hydroxaminolysis of alpha-ketoglutaramate at pH 8
-
-
?
2-oxosuccinamate + H2O
2-oxosuccinate + NH3
-
alpha-ketosuccinamate
-
-
?
2-oxosuccinamate + H2O
2-oxosuccinate + NH3
-
no formation of hydroxamate from alpha-ketosuccinamate
-
-
?
2-oxosuccinamate + H2O
oxaloacetate + NH3
the product of asparagine transamination, 2-oxosuccinamate, can be hydrolyzed by the enzyme omega-amidase to form oxaloacetate and ammonia
-
-
?
2-oxosuccinamate + H2O
oxaloacetate + NH3
the product of asparagine transamination, 2-oxosuccinamate, can be hydrolyzed by the enzyme omega-amidase to form oxaloacetate and ammonia
-
-
?
2-oxosuccinamate + H2O
oxaloacetate + NH3
-
the in vivo substrates are generated by transamination of glutamine and asparagine, respectively
-
-
?
2-oxosuccinamate + H2O
oxaloacetate + NH3
-
the in vivo substrates are generated by transamination of glutamine and asparagine, respectively
-
-
?
2-oxosuccinamate + H2O
oxaloacetate + NH3
-
enzyme activity measured in a 96-well plate format
-
-
?
delta-methyl 2-oxoglutarate + H2O
?
-
delta-methyl alpha-ketoglutarate
-
-
?
gamma-ethyl 2-oxoglutarate + H2O
?
-
gamma-methyl alpha-ketoglutarate
-
-
?
gamma-ethyl 2-oxoglutarate + methylamine
?
-
gamma-ethyl alpha-ketoglutarate
-
-
?
gamma-methyl 2-oxoglutarate + H2O
?
-
gamma-methyl alpha-ketoglutarate
-
-
?
gamma-methyl 2-oxoglutarate + methylamine
?
-
transamidation, very poor
-
-
?
glutaramate + hydroxylamine
glutaryl hydroxamate + NH3
-
amidase activity, hydrolysis proceeds at a slower rate than formation of glutaryl hydroxamate in the presence of hydroxylamine
-
-
?
glutaramate + hydroxylamine
glutaryl hydroxamate + NH3
-
hydroxaminolysis
-
-
?
glutarate + hydroxylamine
glutaryl hydroxamate
-
at pH 5, not at pH 7
-
-
?
glutarate + hydroxylamine
glutaryl hydroxamate
-
acyl-transfer reaction
-
-
?
malate + hydroxylamine
malyl hydroxamate + H2O
-
acyl transfer reaction
-
-
?
malate + hydroxylamine
malyl hydroxamate + H2O
-
acyl transfer reaction
-
-
?
methyl glutarate + hydoxylamine
glutaramate + methanol
-
hydroxaminolysis
-
-
?
N-methyl-2-oxoglutaramate + H2O
2-oxoglutarate + methylamine
-
at a slow rate
-
?
N-methyl-2-oxoglutaramate + H2O
2-oxoglutarate + methylamine
-
N-methyl-alpha-ketoglutaramate
-
?
succinamate + H2O
succinic acid + NH3
-
amidase activity, hydrolysis proceeds at a slower rate than formation of succinyl hydroxamate in the presence of hydroxylamine
-
-
?
succinamate + H2O
succinic acid + NH3
-
amidase activity, hydrolysis proceeds at a slower rate than formation of succinyl hydroxamate in the presence of hydroxylamine
-
-
?
succinamate + H2O
succinic acid + NH3
-
amidase activity, hydrolysis proceeds at a slower rate than formation of succinyl hydroxamate in the presence of hydroxylamine
-
-
?
succinamate + hydroxylamine
succinyl hydroxamate + NH3
-
amide transfer reaction
-
-
r
succinamate + hydroxylamine
succinyl hydroxamate + NH3
-
hydroxaminolysis
-
-
r
succinamate + hydroxylamine
succinyl hydroxamate + NH3
-
catalyzes formation of hydroxamate much more rapidly than hydrolysis of the amide
-
-
r
succinamate + hydroxylamine
succinyl hydroxamate + NH3
-
amide transfer reaction
-
-
r
succinamide + hydroxylamine
succinyl hydroxamate + ?
-
amide transfer reaction
-
-
r
succinamide + hydroxylamine
succinyl hydroxamate + ?
-
amide transfer reaction
-
-
r
succinate + hydroxylamine
succinyl hydroxamate + H2O
-
very high substrate concentration required for acyl transfer reaction
-
-
r
succinate + hydroxylamine
succinyl hydroxamate + H2O
-
acyl transfer reaction
-
-
r
succinate + hydroxylamine
succinyl hydroxamate + H2O
-
at pH 5, not at pH 7
-
-
r
succinate + hydroxylamine
succinyl hydroxamate + H2O
-
very high substrate concentration required for acyl transfer reaction
-
-
r
succinate + hydroxylamine
succinyl hydroxamate + H2O
-
acyl transfer reaction
-
-
r
succinyl hydroxamate + H2O
succinate + hydroxylamine
-
-
-
-
r
additional information
?
-
the recombinant omega-amidase is equally active with 2-oxosuccinamate, 2-oxoglutaramate and 2-hydroxysuccinamate
-
-
?
additional information
?
-
the recombinant omega-amidase is equally active with 2-oxosuccinamate, 2-oxoglutaramate and 2-hydroxysuccinamate
-
-
?
additional information
?
-
-
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
-
-
?
additional information
?
-
-
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
-
-
?
additional information
?
-
-
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
-
-
?
additional information
?
-
-
no reaction with monocarboxylic acids
-
-
?
additional information
?
-
-
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
-
-
?
additional information
?
-
-
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
-
-
?
additional information
?
-
-
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
-
-
?
additional information
?
-
-
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
-
-
?
additional information
?
-
-
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
-
-
?
additional information
?
-
-
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
-
-
?
additional information
?
-
-
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
-
-
?
additional information
?
-
-
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
-
-
?
additional information
?
-
-
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
-
-
?
additional information
?
-
-
pathway for brain glutamine metabolism involves glutamine transaminase-omega-maidase pathway, L-glutamine: 2-oxo-acid amino-transferase, EC 2.6.1.15 and omega-amidodicarboxylate amidohydrolase, EC 3.5.1.3
-
-
?
additional information
?
-
-
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
-
-
?
additional information
?
-
-
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
-
-
?
additional information
?
-
-
L-glutamine and L-asparagine are no substrates at pH 7.2 and pH 8.5
-
-
?
additional information
?
-
-
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
-
-
?
additional information
?
-
-
catalyzes hydrolysis and transfer reactions, enzyme-dicarboxylic acid complex may react either with H2O or hydroxylamine
-
-
?
additional information
?
-
-
no hydrolysis of glutamine, asparagine, N-methyl-alpha-ketoglutaramate, dimethyl glutarate, or the anilides of succinate or glutarate. No hydrolysis of N-methylsuccinamate or N-ethylsuccinamate
-
-
?
additional information
?
-
-
catalyzes hydrolysis and acyl-transfer reactions with amide and monoalkyl esters of alpha-ketoglutarate, glutarate, and succinate
-
-
?
additional information
?
-
-
catalyzes methanolysis, no alcoholysis using ethanol, 2-chloroethanol, and 2,2,2-trifluoroethanol
-
-
?
additional information
?
-
-
hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, gamma-ester, glutarate, succinate, and p-chloro, p-methyl, and unsubstituted phenyl esters of glutarate
-
-
?
additional information
?
-
-
hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, gamma-ester, glutarate, succinate, and p-chloro, p-methyl, and unsubstituted phenyl esters of glutarate
-
-
?
additional information
?
-
-
rate of hydrolysis is decreased proportionally to the increase in hydroxaminolysis
-
-
?
additional information
?
-
-
glutamine, acetamide, propionamide, butyramide, isobutyramide, isovaleramide, oxamate, malonamate, adipamate, oxamide, adipamide, alpha-ketoadipamate, alpha-keto-DL-gamma-methylglutaramate, DL-alpha-aminomalonamate, alpha-methyl-DL-asparagine, DL-gamma-methyl-L-glutamine, alpha-methyl-DL-glutamine, DL-beta-methyl-DL-glutamine, DL-proline, DL-alanine and L-tyrosine amide: no substrates
-
-
?
additional information
?
-
-
no hydrolysis of asparagine and glutamine
-
-
?
additional information
?
-
-
hydrolyzes monomethyl and ethyl esters of alpha-ketoglutarate, delta esters, succinate, and glutarate
-
-
?
additional information
?
-
-
no hydroxaminolysis of delta-methyl glutamate, delta-ethyl glutamate, malonate, glutamate, glutarate, aspartate, succinate, glutamine, asparagine, succinanilate, glutaranilate, alpha-ketoglutaramate N-methylsuccinamate, and N-ethylsuccinamate at pH 8
-
-
?
additional information
?
-
-
hydrolyzes monomethyl and monoethyl esters of alpha-ketoglutarate, delta-ester, glutarate, and succinate, and monopropyl ester of glutarate
-
-
?
additional information
?
-
-
catalytic sequence involves two steps and an acyl enzyme intermediate
-
-
?
additional information
?
-
-
catalyzes hydroxaminolysis and transamidation reactions with esters and amides
-
-
?
additional information
?
-
-
catalyzes hydroxaminolysis and transamidation reactions with esters and amides
-
-
?
additional information
?
-
-
catalyzes hydroxaminolysis and transamidation reactions with esters and amides
-
-
?
additional information
?
-
-
catalyzes hydroxaminolysis and transamidation reactions with esters and amides
-
-
?
additional information
?
-
-
catalyzes hydroxaminolysis and transamidation reactions with esters and amides
-
-
?
additional information
?
-
-
catalyzes hydrolysis and hydroxaminolysis
-
-
?
additional information
?
-
-
catalyzes hydrolysis and hydroxaminolysis
-
-
?
additional information
?
-
-
conversion of glutamine into alpha-ketoglutarate and ammonia is brought about by two distinct enzymes: glutamine transaminase and omega-amidase
-
-
?
additional information
?
-
-
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
-
-
?
additional information
?
-
-
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
-
-
?
additional information
?
-
-
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
-
-
?
additional information
?
-
-
enzyme of glutamine-transaminase-omega-amidase pathway, role in degradation of glutamine
-
-
?
additional information
?
-
-
enzyme, in conjunction with glutamine transaminase comprises enzyme system previously designated glutaminase II, involved in metabolism of glutamine
-
-
?
additional information
?
-
-
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
-
-
?
additional information
?
-
-
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
-
-
?
additional information
?
-
-
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
-
-
?
additional information
?
-
-
second enzyme of the enzyme system capable of converting glutamine to ammonia and capable of generating relatively large amounts of ammonia from glutamine, the glutaminase II pathway, glutamine transaminase, EC 2.6.1.15 plus omega-amidase, EC 3.5.1.3 pathway
-
-
?
additional information
?
-
-
pathway for brain glutamine metabolism involves glutamine transaminase-omega-maidase pathway, L-glutamine: 2-oxo-acid amino-transferase, EC 2.6.1.15 and omega-amidodicarboxylate amidohydrolase, EC 3.5.1.3
-
-
?
additional information
?
-
-
high concentrations of omega-amidase in choroid plexus and in the S1-S3 region of nephron suggest that the glutaminase II pathway may generate appreciable ammonia locally in these organs, perhaps in association with transport
-
-
?
additional information
?
-
-
glutamine-transaminase-omega-amidase pathway, metabolic role: utilization of glutamine, formation of ammonia, ammoniagenesis, and utilization of certain alpha-keto acids for synthesis of corresponding amino acids
-
-
?
additional information
?
-
-
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
-
-
?
additional information
?
-
-
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
-
-
?
additional information
?
-
-
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
-
-
?
additional information
?
-
-
no reaction with monocarboxylic acids
-
-
?
additional information
?
-
-
catalyzes several enzymatic reactions: amide transfer, acyl transfer, and amide hydrolysis, are consistent with acyl enzyme type of intermediate
-
-
?
additional information
?
-
-
glutamine, acetamide, acetate, propionate, aspartate and glutamate not or very poor substrates
-
-
?
additional information
?
-
-
catalyzes hydrolysis of dicarboxylic acid amides and the formation of hydroxamates from amides, amide transfer, of the corresponding acids, acyl transfer
-
-
?
