3.5.1.3: omega-amidase

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For detailed information about omega-amidase, go to the full flat file.

Reaction

Synonyms

alpha-keto acid omega-amidase, alpha-keto acid-omega-amidase, amidase, omega, dicarboxylate omega-amidase, NIT2, Nit2/omega-amidase, nitrilase-like protein 2, omega-amidase, omega-amidase/Nit2, omega-amido dicarboxylate amidohydrolase, omega-amidodicarboxylate amidohydrolase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.3 omega-amidase

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Results	in table
8	Activating Compound
208	AA Sequence
4	Application
1	CAS Registry Number
7	Cloned(Commentary)
3	Cofactor
5	Disease/ Diagnostics
14	General Information
4	General Stability
36	Inhibitors
11	kcat/KM [mM/s]
63	KM Value [mM]
35	Localization
9	Metals/Ions
17	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
4	Organic Solvent Stability
271	Organism
1	Oxidation Stability
9	Pathway
36	pH Optimum
5	pH Stability
15	Purification (Commentary)
10	Reaction
2	Reaction Type
45	Reference
1	Renatured (Commentary)
84	Source Tissue
18	Specific Activity [micromol/min/mg]
5	Storage Stability
272	Substrates and Products (Substrate)
8	Subunits
19	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
11	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.5.1.3 - omega-amidase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, protein concentration above 0.1 mg/ml, 0.05 M potassium phosphate, pH 7.2, 0.05 M KCl, 3 months, quite stable

Bacillus subtilis

-

288886

-20°C, protein concentration above 0.1 mg/ml, 3 months, quite stable

Thermus aquaticus

-

288886

3°C, 1 mM dithiothreitol, 2 weeks, less than 10% activity lost

Rattus norvegicus

-

288892

cytoplasmic and mitochondrial enzyme, 20% glycerol, 5 mM 2-mercaptoethanol, pH 7.2

Rattus norvegicus

-

288884

enzyme with high specific activity, 30% glycerol, 0.8 mg protein/ml, 11 years, still active

Rattus norvegicus

-

288884