3.5.1.3: omega-amidase

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For detailed information about omega-amidase, go to the full flat file.

Word Map on EC 3.5.1.3

3.5.1.3

alpha-ketoglutaramate

asparagine

transamination

ammonia

oxaloacetate

succinamate

glutaminase

transamidase

analysis

medicine

biotechnology

Reaction

Synonyms

alpha-keto acid omega-amidase, alpha-keto acid-omega-amidase, amidase, omega, dicarboxylate omega-amidase, NIT2, Nit2/omega-amidase, nitrilase-like protein 2, omega-amidase, omega-amidase/Nit2, omega-amido dicarboxylate amidohydrolase, omega-amidodicarboxylate amidohydrolase

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.1 In linear amides

3.5.1.3 omega-amidase

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Results	in table
8	Activating Compound
208	AA Sequence
4	Application
1	CAS Registry Number
7	Cloned(Commentary)
3	Cofactor
5	Disease/ Diagnostics
14	General Information
4	General Stability
36	Inhibitors
11	kcat/KM [mM/s]
63	KM Value [mM]
35	Localization
9	Metals/Ions
17	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
4	Organic Solvent Stability
271	Organism
1	Oxidation Stability
9	Pathway
36	pH Optimum
5	pH Stability
15	Purification (Commentary)
10	Reaction
2	Reaction Type
45	Reference
1	Renatured (Commentary)
84	Source Tissue
18	Specific Activity [micromol/min/mg]
5	Storage Stability
272	Substrates and Products (Substrate)
8	Subunits
19	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
11	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 3.5.1.3 - omega-amidase

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0.016

0.51

substrate: L-2-hydroxysuccinamate, pH 7.4, 37°C

0.59

1.4

substrate: L-2-hydroxyglutaramate, pH 7.4, 37°C

10.9

11.7

126

2-oxoglutaramate as substrate, pH 8.5, 37°C

710840

17.4

Rattus norvegicus

ethyl alpha-ketoglutarate hydrolysis

288888

17.67

Rattus norvegicus

liver, mitochondrial enzyme

288884

Homo sapiens

Q9NQR4

substrate: 2-oxoglutaramate, pH 7.4, 37°C

752906

3.4

Homo sapiens

Q9NQR4

substrate: succinamate, pH 7.4, 37°C

752906

42.4

Rattus norvegicus

liver, soluble, cytoplasmic, enzyme

288884

570

Rattus norvegicus

2-oxosuccinamate as substrate, pH 8.5, 37°C

710840

Thermus aquaticus

288886

additional information

4 entries