3.5.1.3: omega-amidase

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For detailed information about omega-amidase, go to the full flat file.

Word Map on EC 3.5.1.3

3.5.1.3

alpha-ketoglutaramate

asparagine

transamination

ammonia

oxaloacetate

succinamate

glutaminase

transamidase

analysis

medicine

biotechnology

Reaction

Synonyms

alpha-keto acid omega-amidase, alpha-keto acid-omega-amidase, amidase, omega, dicarboxylate omega-amidase, NIT2, Nit2/omega-amidase, nitrilase-like protein 2, omega-amidase, omega-amidase/Nit2, omega-amido dicarboxylate amidohydrolase, omega-amidodicarboxylate amidohydrolase

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.1 In linear amides

3.5.1.3 omega-amidase

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Results	in table
8	Activating Compound
208	AA Sequence
4	Application
1	CAS Registry Number
7	Cloned(Commentary)
3	Cofactor
5	Disease/ Diagnostics
14	General Information
4	General Stability
36	Inhibitors
11	kcat/KM [mM/s]
63	KM Value [mM]
35	Localization
9	Metals/Ions
17	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
4	Organic Solvent Stability
271	Organism
1	Oxidation Stability
9	Pathway
36	pH Optimum
5	pH Stability
15	Purification (Commentary)
10	Reaction
2	Reaction Type
45	Reference
1	Renatured (Commentary)
84	Source Tissue
18	Specific Activity [micromol/min/mg]
5	Storage Stability
272	Substrates and Products (Substrate)
8	Subunits
19	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
11	Turnover Number [1/s]

pH Optimum

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pH Optimum on EC 3.5.1.3 - omega-amidase

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Rattus norvegicus

hydroxamate formation, substrate glutarate or succinate

6.5

6.5 - 7.5

6.5 - 9.5

broad pH-optimum, hydrolysis of alpha-ketosuccinamate

7 - 9

7.1

7.2

very low activity on 2-oxoglutaramte as substrate compared to assay at pH 8.5, presumably because Nit2 only acts on the linear forms of this substrate. The generation of the linear form from the predominant, cyclic form is pH-dependent, being much fatsre at pH 8.5 than at pH 7.2

711316

7.4

Rattus norvegicus

ethyl alpha-keto-glutarate hydrolysis, assay at

assay at

8.5

8.5 - 9

pH-optimum for hydroxaminolysis of alpha-ketoglutaramate

288889

2 entries

additional information

5 entries