3.5.1.3: omega-amidase

This is an abbreviated version!
For detailed information about omega-amidase, go to the full flat file.

Word Map on EC 3.5.1.3

3.5.1.3

alpha-ketoglutaramate

asparagine

transamination

ammonia

oxaloacetate

succinamate

glutaminase

transamidase

analysis

medicine

biotechnology

Reaction

Synonyms

alpha-keto acid omega-amidase, alpha-keto acid-omega-amidase, amidase, omega, dicarboxylate omega-amidase, NIT2, Nit2/omega-amidase, nitrilase-like protein 2, omega-amidase, omega-amidase/Nit2, omega-amido dicarboxylate amidohydrolase, omega-amidodicarboxylate amidohydrolase

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.1 In linear amides

3.5.1.3 omega-amidase

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Results	in table
8	Activating Compound
208	AA Sequence
4	Application
1	CAS Registry Number
7	Cloned(Commentary)
3	Cofactor
5	Disease/ Diagnostics
14	General Information
4	General Stability
36	Inhibitors
11	kcat/KM [mM/s]
63	KM Value [mM]
35	Localization
9	Metals/Ions
17	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
4	Organic Solvent Stability
271	Organism
1	Oxidation Stability
9	Pathway
36	pH Optimum
5	pH Stability
15	Purification (Commentary)
10	Reaction
2	Reaction Type
45	Reference
1	Renatured (Commentary)
84	Source Tissue
18	Specific Activity [micromol/min/mg]
5	Storage Stability
272	Substrates and Products (Substrate)
8	Subunits
19	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
11	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 3.5.1.3 - omega-amidase

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120000

Bacillus subtilis

gel filtration

288886

26000

Rattus norvegicus

1 * 31000 + 1 * 26000, or 2 * 31000, not distinguishable, calculated from sequence, the 26000 Da subunit is a glutathione S-transferase subunit, the 31000 Da the catalytic subunit of omega-amidase

711317

27000

Rattus norvegicus

1 * 30000 + 1 * 27000, or 2 * 30000, not distinguishable, SDS-PAGE, the 27000 Da subunit is a glutathione S-transferase subunit, the 30000 Da the catalytic subunit of omega-amidase

711317

28000

Rattus norvegicus

liver, cytoplasmic enzyme, 2 * 28000

30000

31000

34000

x * 40300, about, sequence calculation, x * 34000, recombinant His-tagged enzyme without transit peptide, SDS-PAGe

36000 - 38500

gel filtration

40300

x * 40300, about, sequence calculation, x * 34000, recombinant His-tagged enzyme without transit peptide, SDS-PAGe

734871

58000

3 entries

60000

Rattus norvegicus

gel filtration on Sephadex G-200

711317

62000

Homo sapiens

gel filtration

711317