3.5.1.3: omega-amidase
This is an abbreviated version!
For detailed information about omega-amidase, go to the full flat file.
Word Map on EC 3.5.1.3
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3.5.1.3
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alpha-ketoglutaramate
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asparagine
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transamination
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ammonia
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oxaloacetate
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succinamate
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glutaminase
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transamidase
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analysis
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medicine
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biotechnology
- 3.5.1.3
- alpha-ketoglutaramate
- asparagine
-
transamination
- ammonia
- oxaloacetate
- succinamate
- glutaminase
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transamidase
- analysis
- medicine
- biotechnology
Reaction
Synonyms
alpha-keto acid omega-amidase, alpha-keto acid-omega-amidase, amidase, omega, dicarboxylate omega-amidase, NIT2, Nit2/omega-amidase, nitrilase-like protein 2, omega-amidase, omega-amidase/Nit2, omega-amido dicarboxylate amidohydrolase, omega-amidodicarboxylate amidohydrolase
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Cloned
Cloned on EC 3.5.1.3 - omega-amidase
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expressed as a fusion ptotein with an N-terminal polyHis tag in Escherichia coli and purified to homogeneity on a HisTrap column
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gene At5g12040, DNA and amino acid sequence determination and analysis, expression of N-terminally His-tagged recombinant enzyme lacking the chloroplast transit peptide, commencing at Met63, in Escherichia coli
gene nit, the gene is encoded in the nic-gene cluster located on plasmid pAO1 and flanked by mobile elements
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Nit2-amplified DNA is obtained by PCR of Nit2 cDNA clnoed from human liver cDNA
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