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3.5.1.3: omega-amidase

This is an abbreviated version!
For detailed information about omega-amidase, go to the full flat file.

Word Map on EC 3.5.1.3

Reaction

a monoamide of a dicarboxylate
+
H2O
=
a dicarboxylate
+
NH3

Synonyms

alpha-keto acid omega-amidase, alpha-keto acid-omega-amidase, amidase, omega, dicarboxylate omega-amidase, NIT2, Nit2/omega-amidase, nitrilase-like protein 2, omega-amidase, omega-amidase/Nit2, omega-amido dicarboxylate amidohydrolase, omega-amidodicarboxylate amidohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.3 omega-amidase

Cloned

Cloned on EC 3.5.1.3 - omega-amidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a fusion ptotein with an N-terminal polyHis tag in Escherichia coli and purified to homogeneity on a HisTrap column
-
gene At5g12040, DNA and amino acid sequence determination and analysis, expression of N-terminally His-tagged recombinant enzyme lacking the chloroplast transit peptide, commencing at Met63, in Escherichia coli
gene nit, the gene is encoded in the nic-gene cluster located on plasmid pAO1 and flanked by mobile elements
-
Nit2-amplified DNA is obtained by PCR of Nit2 cDNA clnoed from human liver cDNA
-
the gene is encoded in the nic-gene cluster on the chromosome
-