3.4.24.40: serralysin
This is an abbreviated version!
For detailed information about serralysin, go to the full flat file.
Word Map on EC 3.4.24.40
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3.4.24.40
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elastase
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metalloproteases
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achromobacter
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lyticus
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caseinolytic
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chrysanthemi
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metzincins
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medicine
- 3.4.24.40
- elastase
- metalloproteases
- achromobacter
- lyticus
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caseinolytic
- chrysanthemi
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metzincins
- medicine
Reaction
Preferential cleavage of bonds with hydrophobic residues in P1' =
Synonyms
aeruginolysin, Alkaline protease, AMP-P, AP, APR, AprA, AprX, arazyme, calcium-regulated alkaline protease, DR2310 protease, Escherichia freundii proteinase, Extracellular metalloproteinase, LupA, More, protease B, protease C, protease I, Proteinase, Serratia marcescens metallo-, PrtA, PrtA metalloprotease, PrtC, pseudomonal serralysin, Pseudomonas aeruginosa alk. protease, Pseudomonas aeruginosa alkaline protease, Pseudomonas aeruginosa alkaline proteinase, Pseudomonas alkaline protease, psychrophilic alkaline metalloprotease, serralysin, serralysin-like metalloprotease, Serratia marcescens extracellular proteinase, Serratia marcescens metalloprotease, Serratia marcescens metalloproteinase, SlpB, SMP, thermoalkaline protease, thermostable alkaline protease, zinc proteinase
ECTree
3.4.24.40 serralysinAdvanced search results
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results (Inhibitors
Inhibitors on EC 3.4.24.40 - serralysin
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
Ac-Val-Leu-Lys-4-mercaptoanilide
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pH 7.8, 37°C, 0.2 mM 69% inhibition, 0.3 mM complete inhibition
APRin protein
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slow binding inhibition, reversible inhibition. and truncated mutants
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benzyloxycarbonyl-D-Ala-Leu-Ala
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competitive to benzyloxycarbonyl-Ala-Phe-Gly-Ala
Benzyloxycarbonyl-Gly-Leu-D-Ala
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benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate
Benzyloxycarbonyl-Gly-Leu-Gly
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benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate
Benzyloxycarbonyl-Gly-Leu-NH2
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benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate
Benzyloxycarbonyl-Phe-D-Leu-Ala
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competitive to benzyloxycarbonyl-Ala-Phe-Gly-Ala
Bovine pancreatic trypsin inhibitor
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0.01 mM, 5% inhibition at pH 5.5
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diisopropylfluorophosphate
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1 mM, weak inhibition at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+
Elastatinal
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0.1 mM, 11% inhibition at pH 8.0 and without 1 mM Co2+ addition
L-trans-epoxysuccinyl-leucyl-amido-(4-guanido)butane
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0.1 mM, 26% inhibition at pH 8.0 and 1 mM Co2+ addition, 26% inhibition at pH 5.5 without addition of 1 mM Co2+
N-tosyl-L-phenylalanine chloromethyl ketone
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0.1 mM, 18% inhibition at pH 8.0
phenyl-methanesulfonyl fluoride
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1 mM, 14-17% inhibition at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+
phosphoramidon
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0.1 mM, no inhibition at pH 8.0 and 1 mM Co2+ addition, 25% inhibition at pH 8.0 and 5.5 without addition of 1 mM Co2+
1,10-phenanthroline
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30 min, room temperature, pH 7.5, complete and irreversible inhibition in a concentration range below 1 mM
1,10-phenanthroline
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pH 7.8, 37°C, 1 mM 66% inhibition, 3mM 90% inhibition
1,10-phenanthroline
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10 mM, strong inhibition at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+
1,10-phenanthroline
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reversible by dialysis, Mg2+ or Co2+ partially restores; strong
1,10-phenanthroline
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activity can be restored with the addition of Mn2+, Cu2+ and Co2+ up to 90-200% of its original value, while Zn2+ is inefficient
EDTA
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30 min, room temperature, pH 7.5, complete inhibition in a concentration range below 1 mM
EDTA
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10 mM, almost complete inactivation at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+
EDTA
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Ca2+ (partially); Cu2+ restores partially; more effective at acidic pH; partially reversible by dialysis
EDTA
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Ca2+ (MW 56000 and MW 60000 enzyme); Ca2+ (partially); Co2+ (Serratia sp. E-16 enzyme); Cu2+ (not); Fe2+ (only MW 56000 enzyme); Mg2+ (not); Serratia enzymes; Zn2+ (MW 56000 and MW 60000 enzyme)
EDTA
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Ca2+ (restored activity higher than native activity); enhances inhibition by guanidine-HCl; Fe2+ (partially); Mg2+; Mn2+ (restored activity higher than native activity); no reactivation by K+; Zn2+ (restored activity higher than native activity)
EDTA
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strong inhibition with 2.6% residual activity at 10 mM, enzyme activity can be regained by the addition of Co2+, Cu2+, Fe2+, Mg2+, and Zn2+ ions after chelation of ions with EDTA
EDTA
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inhibits the activity only partially even either after long incubation or in excess amount
leupeptin
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0.1 mM, 10% inhibition at pH 8.0 and 1 mM Co2+ addition, 7% inhibition at pH 8.0 without 1 mM Co2+ addition
N-ethylmaleimide
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0.1 mM, 14% inhibition at pH 8.0 and 1 mM Co2+ addition
Periplasmic endogen inhibitor peptide of Serratia marcescens
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recombinant and native form, pH- and heat-stable protein, inhibition at 1:1 molar ratio, highly specific for Serratia marcescens proteinase, mechanism
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Zn2+
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both the Zn2+ inhibition of protease B activity and its resistance to EDTA inhibition might be caused by an Asp in position 191 where most of the serralysins contain Asn
additional information
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2-mercaptoethanol; diisopropyl phosphofluoridate; iodine, ovomucoid; monoiodoacetic acid; PCMB; potato inhibitor; soybean trypsin inhibitor
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additional information
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diisopropyl phosphofluoridate; PCMB; potato inhibitor; soybean trypsin inhibitor; tosyl-L-Phe chloromethyl ketone or tosyl-L-Lys chloromethyl ketone
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additional information
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citrate, NaF, oxalate; diisopropyl phosphofluoridate; monoiodoacetic acid; PCMB; potato inhibitor; soybean trypsin inhibitor
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additional information
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not inhibited by chloromethylketone or hydroxamate
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additional information
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Lima bean, human pancreatic or egg white trypsin inhibitor, human alpha1-antitrypsin; no inhibition by Mg2+, Mn2+, Ca2+, Ba2+, NaCN, NaN3, sodium arsenite, p-hydroxymercuribenzoate; soybean trypsin inhibitor
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additional information
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2-mercaptoethanol (Serratia marcescens); diisopropyl phosphofluoridate (Serratia marcescens); GSH, EGTA (Serratia marcescens), phosphoramidon, zincov (i.e. 2-(N-hydroxycarboxamido)-4-methylpentanoyl-L-Ala-glycinamide, thermolysin inhibitor); monoiodoacetic acid (Serratia marcescens)
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additional information
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not inhibited by iodoacetic acid and phenylmethylsulfonyl fluoride
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additional information
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not inhibitory: pepstatin (0.0015 mM), tosyllysyl chloromethyl ketone (0.05 mM), tosylphenylalanyl chloromethyl ketone (0.05 mM), tiorphan (0.004 mM), Triton X-100 and Tween up to 0.1% (v/v)
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