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Literature summary for 3.4.24.40 extracted from

  • Shibuya, Y.; Yamamoto, T.; Morimoto, T.; Nishino, N.; Kambara, T.; Okabe, H.
    Pseudomonas aeruginosa alkaline proteinase might share a biological function with plasmin (1991), Biochim. Biophys. Acta, 1077, 316-324.
    View publication on PubMed
Search for more literature for 3.4.24.40

Application

Application Comment Organism
medicine anti-coagulant activity in human plasma Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline pH 7.8, 37°C, 1 mM 66% inhibition, 3mM 90% inhibition Pseudomonas aeruginosa
Ac-Val-Leu-Lys-4-mercaptoanilide pH 7.8, 37°C, 0.2 mM 69% inhibition, 0.3 mM complete inhibition Pseudomonas aeruginosa
additional information not inhibited by chloromethylketone or hydroxamate Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide pH 7.8, 37°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HPLC Pseudomonas aeruginosa

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fibrin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
Fibrinogen + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
Suc-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
t-butyloxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
t-butyloxycarbonyl-Met-Thr-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
t-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
t-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?