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Ca2+
-
-
Ca2+
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in presence of Ca2+, Zn2+ or Co2+ may recativate EDTA-inactivated protease. Ca2+ concentrations required are between 0.01 mM and 0.1 mM, inhibitory above 0.1 mM
Ca2+
-
no activation by added Ca2+
Ca2+
-
1-2 gatom/mol enzyme
Ca2+
-
calcium-regulated alkaline protease, role of Ca2+ in enzyme folding and activation: Ca2+binding induces RTX folding, which serves to chaperone the folding of the protease domain. Subsequent association of the RTX domain with an N-terminal alpha-helix stabilizes AP, Ca2+ regulates the RTX family of virulence factors, overview
Ca2+
-
Ca2+ leads to an increase in protease productivity by 300%
Ca2+
-
weakly restores activity after EDTA treatment, Serratia marcescens MW 56000 and MW 60000 enzymes
Ca2+
-
7 gatom/mol enzyme
Co2+
-
activation
Co2+
-
1 mM, at pH 10, not at pH 7
Co2+
-
in the presence of Ca2+, restoration of EDTA-inactivied protease. No reactivation in absence of Ca2+
Co2+
-
above 5 mM, benzoxycarbonyl-Ala-Phe-Gly-Ala as substrate
Co2+
-
120.1% activity at 5 mM
Co2+
-
slightly activating
Cu2+
-
activates
Cu2+
-
slightly activating
Fe2+
-
restores activity after EDTA treatment, Serratia marcescens MW 56000 enzyme, not MW 60000 enzyme
Fe2+
-
10 mM, 197% of initial activity
Mg2+
-
activation
Zn2+
-
zinc-containing metalloproteinase
Zn2+
-
in the presence of Ca2+, restoration of EDTA-inactivied protease. No reactivation in absence of Ca2+. Inhibitory above 0.025 mM
Zn2+
-
zinc-binding consensus sequence in comparison to astacins, snake venom, matrix metalloproteinases
Zn2+
-
zinc-containing metalloproteinase (not)
Zn2+
-
zinc-binding motif: His-Glu-Xaa-Xaa-His
Zn2+
-
zinc-binding motif: His-Glu-Xaa-Xaa-His (Pseudomonas aeruginosa, Erwinia chrysanthemi, Serratia sp. E-15)
Zn2+
-
zinc-containing metalloproteinase
Zn2+
-
zinc-binding consensus sequence in comparison to astacins, snake venom, matrix metalloproteinases
Zn2+
-
zinc-containing metalloproteinase (not)
Zn2+
-
zinc-binding motif: His-Glu-Xaa-Xaa-His
Zn2+
-
zinc-binding motif: His-Glu-Xaa-Xaa-His (Pseudomonas aeruginosa, Erwinia chrysanthemi, Serratia sp. E-15)
Zn2+
-
zinc-containing metalloproteinase
Zn2+
-
zinc-dependent metalloprotease
Zn2+
-
Zn2+ is bound at the active site
Zn2+
-
one Zn2+ per protein molecule
Zn2+
-
added Zn2+ inhibits
Zn2+
-
restores activity after EDTA treatment (Serratia marcescens MW 56000 and MW 60000 enzymes)
Zn2+
-
1 gatom/mol enzyme (Serratia marcescens, Serratia sp., Serratia piscatrum)
Zn2+
-
zinc-binding consensus sequence in comparison to astacins, snake venom, matrix metalloproteinases
Zn2+
-
zinc-binding motif: His-Glu-Xaa-Xaa-His
Zn2+
-
zinc-containing metalloproteinase
Zn2+
-
114.02% activity at 5 mM
Zn2+
-
1 gatom/mol enzyme (Serratia marcescens, Serratia sp., Serratia piscatrum)
Zn2+
-
zinc-binding motif: His-Glu-Xaa-Xaa-His
Zn2+
-
zinc-binding motif: His-Glu-Xaa-Xaa-His (Pseudomonas aeruginosa, Erwinia chrysanthemi, Serratia sp. E-15)
Zn2+
-
zinc-containing metalloproteinase
additional information
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atomic absorption spectroscopy
additional information
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magnesium, manganese, cobalt, copper or iron
additional information
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no activation by Rb2+
additional information
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contains no significant amount of any metal except Ca2+
additional information
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no activation by Mn2+
additional information
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no activation by Ni2+
additional information
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no activation by Fe3+, Mg2+
additional information
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no activation by Mn2+
additional information
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not influenced by Ba2+, Ca2+, Cu2+, K+, Mg2+, Na+, and Ni2+