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Literature summary for 3.4.24.40 extracted from
- Serralysin and related bacterial proteinases (1995), Methods Enzymol., 248, 395-413.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| Pseudomonas aeruginosa | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Citrobacter freundii |  |
| 1,10-phenanthroline | - |
Pseudomonas aeruginosa | |
| 1,10-phenanthroline | Serratia marcescens enzyme | Serratia marcescens | |
| antipain | only Serratia marcescens MW 56000 enzyme | Serratia marcescens | |
| Cysteine hydrochloride | not Serratia marcescens enzyme | Citrobacter freundii | |
| Cysteine hydrochloride | not Serratia marcescens enzyme | Dickeya chrysanthemi | |
| Cysteine hydrochloride | not Serratia marcescens enzyme | Proteus mirabilis | |
| Cysteine hydrochloride | not Serratia marcescens enzyme | Pseudomonas aeruginosa | |
| Cysteine hydrochloride | not Serratia marcescens enzyme | Pseudomonas fragi | |
| Cysteine hydrochloride | not Serratia marcescens enzyme | Serratia marcescens | |
| Cysteine hydrochloride | not Serratia marcescens enzyme | Serratia sp. E-15 | |
| DTT | weak, Serratia marcescens | Citrobacter freundii | |
| DTT | weak, Serratia marcescens | Dickeya chrysanthemi | |
| DTT | weak, Serratia marcescens | Proteus mirabilis | |
| DTT | weak, Serratia marcescens | Pseudomonas aeruginosa | |
| DTT | weak, Serratia marcescens | Pseudomonas fragi | |
| DTT | weak, Serratia marcescens | Serratia marcescens | |
| DTT | weak, Serratia marcescens | Serratia sp. E-15 | |
| EDTA | Mn2+ | Citrobacter freundii | |
| EDTA | - |
Dickeya chrysanthemi | |
| EDTA | Mn2+ | Pseudomonas aeruginosa | |
| EDTA | Ca2+ (MW 56000 and MW 60000 enzyme); Ca2+ (partially); Co2+ (Serratia sp. E-16 enzyme); Cu2+ (not); Fe2+ (only MW 56000 enzyme); Mg2+ (not); Serratia enzymes; Zn2+ (MW 56000 and MW 60000 enzyme) | Serratia marcescens | |
| leupeptin | weak, only Serratia marcescens MW 56000 enzyme | Serratia marcescens | |
| additional information | - |
Citrobacter freundii | |
| additional information | - |
Pseudomonas aeruginosa | |
| additional information | 2-mercaptoethanol (Serratia marcescens); diisopropyl phosphofluoridate (Serratia marcescens); GSH, EGTA (Serratia marcescens), phosphoramidon, zincov (i.e. 2-(N-hydroxycarboxamido)-4-methylpentanoyl-L-Ala-glycinamide, thermolysin inhibitor); monoiodoacetic acid (Serratia marcescens) | Serratia marcescens | |
| N-ethylmaleimide | not (Serratia marcescens enzyme) | Citrobacter freundii | |
| N-ethylmaleimide | not (Serratia marcescens enzyme) | Dickeya chrysanthemi | |
| N-ethylmaleimide | not (Serratia marcescens enzyme) | Proteus mirabilis | |
| N-ethylmaleimide | not (Serratia marcescens enzyme) | Pseudomonas aeruginosa | |
| N-ethylmaleimide | not (Serratia marcescens enzyme) | Pseudomonas fragi | |
| N-ethylmaleimide | not (Serratia marcescens enzyme) | Serratia marcescens | |
| N-ethylmaleimide | not (Serratia marcescens enzyme) | Serratia sp. E-15 | |
| PMSF | weak, Serratia marcescens | Serratia marcescens | |
| Tetramethylenepentamine | Serratia marcescens | Serratia marcescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
Benzyloxycarbonyl-Phe-Ala-Ala | - |
Pseudomonas aeruginosa | |
| 0.6 | - |
benzyloxycarbonyl-Gly-Leu-Phe | benzyloxycarbonyl-Ala-Phe-Leu-Ala | Pseudomonas aeruginosa | |
| 2.5 | - |
benzyloxycarbonyl-Val-Ala-Ala | benzyloxycarbonyl-Ala-Phe-Gly-Ala | Pseudomonas aeruginosa | |
| 2.7 | - |
Benzyloxycarbonyl-Ala-Ala-Ala | - |
Pseudomonas aeruginosa | |
| 4.3 | - |
Benzyloxycarbonyl-Gly-Leu-Gly-Gly-Ala | - |
Pseudomonas aeruginosa | |
| 5.3 | - |
Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala | - |
Pseudomonas aeruginosa | |
| 5.4 | - |
benzyloxycarbonyl-Gly-Gly-Leu-Ala | benzyloxycarbonyl-Ala-Gly-Gly-Leu | Pseudomonas aeruginosa | |
| 7.7 | - |
Benzyloxycarbonyl-Gly-Ala-Ala | - |
Pseudomonas aeruginosa | |
| 11 | - |
Benzyloxycarbonyl-Gly-Leu-Gly-Gly | - |
Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas aeruginosa | - |
- |
| extracellular | - |
Serratia marcescens | - |
- |
| extracellular | - |
Proteus mirabilis | - |
- |
| extracellular | - |
Pseudomonas fragi | - |
- |
| extracellular | - |
Dickeya chrysanthemi | - |
- |
| extracellular | - |
Citrobacter freundii | - |
- |
| extracellular | - |
Serratia sp. E-15 | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Pseudomonas aeruginosa | |
| Ca2+ | - |
Dickeya chrysanthemi | |
| Ca2+ | - |
Citrobacter freundii | |
| Ca2+ | weakly restores activity after EDTA treatment, Serratia marcescens MW 56000 and MW 60000 enzymes | Serratia marcescens | |
| Co2+ | activation | Pseudomonas aeruginosa | |
| Co2+ | activation | Serratia marcescens | |
| Co2+ | activation | Citrobacter freundii | |
| Fe2+ | restores activity after EDTA treatment, Serratia marcescens MW 56000 enzyme, not MW 60000 enzyme | Serratia marcescens | |
| Zn2+ | restores activity after EDTA treatment (Serratia marcescens MW 56000 and MW 60000 enzymes) | Serratia marcescens | |
| Zn2+ | 1 gatom/mol enzyme (Serratia marcescens, Serratia sp., Serratia piscatrum) | Serratia marcescens | |
| Zn2+ | 1 gatom/mol enzyme (Serratia marcescens, Serratia sp., Serratia piscatrum) | Serratia sp. E-15 | |
| Zn2+ | 1 mol/mol enzyme | Serratia marcescens | |
| Zn2+ | 1 mol/mol enzyme | Serratia sp. E-15 | |
| Zn2+ | zinc-binding motif: His-Glu-Xaa-Xaa-His | Pseudomonas aeruginosa | |
| Zn2+ | zinc-binding motif: His-Glu-Xaa-Xaa-His | Serratia marcescens | |
| Zn2+ | zinc-binding motif: His-Glu-Xaa-Xaa-His | Dickeya chrysanthemi | |
| Zn2+ | zinc-binding motif: His-Glu-Xaa-Xaa-His | Serratia sp. E-15 | |
| Zn2+ | zinc-binding motif: His-Glu-Xaa-Xaa-His (Pseudomonas aeruginosa, Erwinia chrysanthemi, Serratia sp. E-15) | Pseudomonas aeruginosa | |
| Zn2+ | zinc-binding motif: His-Glu-Xaa-Xaa-His (Pseudomonas aeruginosa, Erwinia chrysanthemi, Serratia sp. E-15) | Dickeya chrysanthemi | |
| Zn2+ | zinc-binding motif: His-Glu-Xaa-Xaa-His (Pseudomonas aeruginosa, Erwinia chrysanthemi, Serratia sp. E-15) | Serratia sp. E-15 | |
| Zn2+ | zinc-containing metalloproteinase | Pseudomonas aeruginosa | |
| Zn2+ | zinc-containing metalloproteinase | Serratia marcescens | |
| Zn2+ | zinc-containing metalloproteinase | Dickeya chrysanthemi | |
| Zn2+ | zinc-containing metalloproteinase | Citrobacter freundii | |
| Zn2+ | zinc-containing metalloproteinase | Serratia sp. E-15 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
primary structure of Serratia sp., Erwinia chrysanthemi and Pseudomonas aeruginosa enzyme, tertiary structure of Serratia sp. and Pseudomonas aeruginosa enzyme | Pseudomonas aeruginosa |
| additional information | - |
primary structure of Serratia sp., Erwinia chrysanthemi and Pseudomonas aeruginosa enzyme, tertiary structure of Serratia sp. and Pseudomonas aeruginosa enzyme | Serratia marcescens |
| additional information | - |
primary structure of Serratia sp., Erwinia chrysanthemi and Pseudomonas aeruginosa enzyme, tertiary structure of Serratia sp. and Pseudomonas aeruginosa enzyme | Dickeya chrysanthemi |
| additional information | - |
primary structure of Serratia sp., Erwinia chrysanthemi and Pseudomonas aeruginosa enzyme, tertiary structure of Serratia sp. and Pseudomonas aeruginosa enzyme | Citrobacter freundii |
| additional information | - |
primary structure of Serratia sp., Erwinia chrysanthemi and Pseudomonas aeruginosa enzyme, tertiary structure of Serratia sp. and Pseudomonas aeruginosa enzyme | Serratia sp. E-15 |
| 49500 | - |
Pseudomonas aeruginosa, deduced from nucleotide sequence | Pseudomonas aeruginosa |
| 50600 | - |
Serratia piscatrum, deduced from nucleotide sequence | Serratia sp. E-15 |
| 56000 | - |
Serratia marcescens, strain kums, two enzymes: MW 56000 and MW 60000 | Serratia marcescens |
| 60000 | - |
Serratia marcescens, two enzymes: MW 56000 and MW 60000 | Serratia marcescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
- |
- |
| Dickeya chrysanthemi | - |
plant pathogen | - |
| Proteus mirabilis | - |
- |
- |
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas fragi | - |
- |
- |
| Serratia marcescens | - |
strain kums 3958 (clinical isolate) | - |
| Serratia sp. E-15 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
| 2 immunologically not related enzymes: MW 56000 and MW 60000 | Serratia marcescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas aeruginosa |
| additional information | - |
- |
Serratia marcescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, lyophilized Serratia marcescens enzyme, several months | Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Aminobenzoyl-Gly-Phe-Arg-Leu-Leu 4-nitrobenzyl amide + H2O | Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme | Pseudomonas aeruginosa | ? | - |
? | |
| 2-Aminobenzoyl-Gly-Phe-Arg-Leu-Leu 4-nitrobenzyl amide + H2O | Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme | Serratia marcescens | ? | - |
? | |
| 2-Aminobenzoyl-Gly-Phe-Arg-Xaa 4-nitrobenzyl amide + H2O | Xaa is Gly, Ala, Val, Leu or Phe, Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme | Pseudomonas aeruginosa | ? | - |
? | |
| 2-Aminobenzoyl-Gly-Phe-Arg-Xaa 4-nitrobenzyl amide + H2O | Xaa is Gly, Ala, Val, Leu or Phe, Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme | Serratia marcescens | ? | - |
? | |
| Azocoll + H2O | - |
Serratia marcescens | ? | - |
? | |
| Benzyloxycarbonyl-Ala-Ala-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Ala-Gly-Gly-Leu + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Ala-Gly-Gly-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Ala-Phe-Gly-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Ala-Phe-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Ala-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| benzyloxycarbonyl-Gly-Gly-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Leu-Gly-Gly + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Leu-Gly-Gly-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Leu-Phe + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Phe-Ala-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O | Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme | Pseudomonas aeruginosa | Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O | Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme | Serratia marcescens | Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Val-Ala-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| casein + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| casein + H2O | - |
Serratia marcescens | ? | - |
? | |
| casein + H2O | - |
Proteus mirabilis | ? | - |
? | |
| casein + H2O | - |
Pseudomonas fragi | ? | - |
? | |
| casein + H2O | - |
Dickeya chrysanthemi | ? | - |
? | |
| casein + H2O | - |
Citrobacter freundii | ? | - |
? | |
| casein + H2O | - |
Serratia sp. E-15 | ? | - |
? | |
| Gelatin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Gelatin + H2O | - |
Dickeya chrysanthemi | ? | - |
? | |
| Immunoglobulin A1 + H2O | Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region | Serratia marcescens | ? | - |
? | |
| Immunoglobulin A2 + H2O | Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region | Serratia marcescens | ? | - |
? | |
| Immunoglobulin G1 + H2O | Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region | Serratia marcescens | ? | - |
? | |
| Immunoglobulin G2 + H2O | Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region | Serratia marcescens | ? | - |
? | |
| Immunoglobulin G3 + H2O | Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region | Serratia marcescens | ? | - |
? | |
| Immunoglobulin G4 + H2O | Serratia marcescens, MW 56000 enzyme, predominantly interdomain cleavage at hinge region | Serratia marcescens | ? | - |
? | |
| additional information | - |
Serratia marcescens | ? | - |
? | |
| additional information | elastin | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | broad substrate side-chain specificity: hydrophobic amino acid residues at P2 and P2' are favorable | Pseudomonas aeruginosa | ? | - |
? | |
| Oxidized insulin B-chain + H2O | cleavage specificity of Pseudomonas aeruginosa, Pseudomonas fragi, Serratia sp. and Proteus mirabilis enzyme | Pseudomonas aeruginosa | ? | - |
? | |
| Oxidized insulin B-chain + H2O | cleavage specificity of Pseudomonas aeruginosa, Pseudomonas fragi, Serratia sp. and Proteus mirabilis enzyme | Proteus mirabilis | ? | - |
? | |
| Oxidized insulin B-chain + H2O | cleavage specificity of Pseudomonas aeruginosa, Pseudomonas fragi, Serratia sp. and Proteus mirabilis enzyme | Pseudomonas fragi | ? | - |
? | |
| Oxidized insulin B-chain + H2O | cleavage specificity of Pseudomonas aeruginosa, Pseudomonas fragi, Serratia sp. and Proteus mirabilis enzyme | Serratia sp. E-15 | ? | - |
? | |
| Plasma fibronectin + H2O | Serratia marcescens MW 52000 enzyme, cleavage sites | Serratia marcescens | ? | - |
? | |
| Plasma fibronectin + H2O | from pig | Serratia marcescens | ? | - |
? | |
| Pro-Phe-Arg 4-methylcoumarin 7-amide + H2O | Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme | Pseudomonas aeruginosa | Pro-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| Pro-Phe-Arg 4-methylcoumarin 7-amide + H2O | Pseudomonas aeruginosa alkaline proteinase and MW 52000 Serratia enzyme | Serratia marcescens | Pro-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | belongs to serralysin subfamily M12 | Pseudomonas aeruginosa |
| More | belongs to serralysin subfamily M12 | Serratia marcescens |
| More | belongs to serralysin subfamily M12 | Proteus mirabilis |
| More | belongs to serralysin subfamily M12 | Pseudomonas fragi |
| More | belongs to serralysin subfamily M12 | Dickeya chrysanthemi |
| More | belongs to serralysin subfamily M12 | Citrobacter freundii |
| More | belongs to serralysin subfamily M12 | Serratia sp. E-15 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | broad | Pseudomonas aeruginosa |
| 6 | 10 | broad | Serratia marcescens |
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